重组Anti-ENAH/MENA抗体[EPR5660] (ab124685)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (ab124685)](https://www.abcam.cn/ps/products/124/ab124685/Images/ab124685-138440-anti-enah-antibody-epr5660-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (ab124685)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5660] to ENAH/MENA
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ENAH/MENA抗体[EPR5660]
参阅全部 ENAH/MENA 一抗 -
描述
兔单克隆抗体[EPR5660] to ENAH/MENA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse
不与反应:
Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- MDA-MB-435, MCF7, HUVEC and A431 cell lysates; Human Uterus tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5660 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa).
|
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen rerieval is recommended. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa). |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen rerieval is recommended. |
应用说明
Is unsuitable for ICC/IF or IP.
靶标
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功能
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. ENAH induces the formation of F-actin rich outgrowths in fibroblasts. Acts synergistically with BAIAP2-alpha and downstream of NTN1 to promote filipodia formation. Required for the actin-based mobility of Listeria monocytogenes. -
组织特异性
Expressed in myoepithelia of parotid, breast, bronchial glands and sweat glands. Expressed in colon-rectum muscolaris mucosae epithelium, pancreas acinar ductal epithelium, endometrium epithelium, prostate fibromuscolar stroma and placenta vascular media. Overexpressed in a majority of breast cancer cell lines and primary breast tumor lesions. -
序列相似性
Belongs to the Ena/VASP family.
Contains 1 WH1 domain. -
结构域
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization. -
翻译后修饰
NTN1-induced PKA phosphorylation on Ser-265 directly parallels the formation of filopodial protrusions.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > filopodium. Cell junction > synapse. Cell junction > focal adhesion. Targeted to the leading edge of lamellipodia and filopodia by MRL family members. Colocalizes at filopodial tips with a number of other proteins including vinculin and zyxlin. Colocalizes with N-WASP at the leading edge. Colocalizes with GPHN and PFN at synapses. - Information by UniProt
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数据库链接
- Entrez Gene: 100720009 Human
- Entrez Gene: 513095 Human
- Entrez Gene: 55740 Human
- Entrez Gene: 13800 Mouse
- Omim: 609061 Human
- SwissProt: Q8N8S7 Human
- SwissProt: Q03173 Mouse
- Unigene: 497893 Human
see all -
别名
- ENA antibody
- Enah antibody
- ENAH_HUMAN antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENAH/MENA antibody [EPR5660] (ab124685)
ab124685, at 1/500 dilution, staining ENAH/MENA in formalin-fixed, paraffin-embedded Human uterus tissue, by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ENAH/MENA antibody [EPR5660] (ab124685)](https://www.abcam.cn/ps/products/124/ab124685/Images/ab124685-138439-anti-enah-antibody-epr5660-western-blot.jpg)
Western blot - Anti-ENAH/MENA antibody [EPR5660] (ab124685)All lanes : Anti-ENAH/MENA antibody [EPR5660] (ab124685) at 1/1000 dilution
Lane 1 : MDA-MB-435 (Human ductal carcinoma cell line) cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 67 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Anti-ENAH/MENA antibody [EPR5660] (ab124685)](https://www.abcam.cn/ps/products/124/ab124685/Images/ab124685-3-benefits-of-recombinant-antibodies.png)
Anti-ENAH/MENA antibody [EPR5660] (ab124685)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124685 被引用在 4 文献中.
- Wang J et al. Circ_0003340 regulates the expression of ENAH to affect the development of esophageal cancer through miR-874-3p. Thorac Cancer 14:815-826 (2023). PubMed: 36737402
- Lu B et al. LncRNA ZFAS1 promotes laryngeal cancer progression through RBFOX2-mediated MENA alternative splicing. Environ Toxicol 38:522-533 (2023). PubMed: 36336961
- He Z et al. Human umbilical cord mesenchymal stem cells-derived exosomes deliver microRNA-375 to downregulate ENAH and thus retard esophageal squamous cell carcinoma progression. J Exp Clin Cancer Res 39:140 (2020). PubMed: 32698859
- Li CF et al. Characterization of gene amplification-driven SKP2 overexpression in myxofibrosarcoma: potential implications in tumor progression and therapeutics. Clin Cancer Res 18:1598-610 (2012). PubMed: 22322669