重组Anti-EMILIN1抗体[EPR14678] - N-terminal (ab185953)
![Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)](https://www.abcam.cn/ps/products/185/ab185953/Images/ab185953-219371-anti-emilin1-antibody-epr14678-n-terminal-western-blot.jpg "Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14678] to EMILIN1 - N-terminal
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EMILIN1抗体[EPR14678] - N-terminal
参阅全部 EMILIN1 一抗 -
描述
兔单克隆抗体[EPR14678] to EMILIN1 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human uterus, fetal kidney and colon cancer tissue lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14678 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab185953于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/30 - 1/50.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 107 kDa).
|
| 说明 |
|---|
|
IP
1/30 - 1/50. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 107 kDa). |
靶标
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功能
May be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. Has cell adhesive capacity. -
组织特异性
Distributed in tissues where resilience and elastic recoil are prominent. Highest levels in the adult small intestine, aorta, lung, uterus, and appendix and in the fetal spleen, kidney, lung, and heart; intermediate expression was detected in adult liver, ovary, colon, stomach, lymph node and spleen; adult heart, bladder, prostate, adrenal gland, mammary gland, placenta and kidney showed low expression whereas a series of other adult tissues, including skeletal muscle and different regions of adult brain show no expression. -
序列相似性
Contains 1 C1q domain.
Contains 1 collagen-like domain.
Contains 1 EMI domain. -
细胞定位
Secreted > extracellular space > extracellular matrix. Found mainly at the interface between amorphous elastin and microfibrils. - Information by UniProt
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数据库链接
- Entrez Gene: 11117 Human
- Omim: 130660 Human
- SwissProt: Q9Y6C2 Human
- Unigene: 63348 Human
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别名
- Elastin microfibril interface located protein 1 antibody
- Elastin microfibril interface located protein antibody
- Elastin microfibril interface-located protein 1 antibody
see all
图片
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Western blot - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)All lanes : Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953) at 1/50000 dilution
Lane 1 : Human uterus tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human colon cancer tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 107 kDa -
![Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)](https://www.abcam.cn/ps/products/185/ab185953/Images/ab185953-219372-anti-emilin1-antibody-epr14678-n-terminal-immunoprecipitation.jpg)
Immunoprecipitation - Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)Western blot analysis of immunoprecipitation pellet from Human fetal kidney tissue lysate labeling EMILIN1 with ab185953 at a 1/50 dilution. Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, at a 1/1000 dilution.
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![Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)](https://www.abcam.cn/ps/products/185/ab185953/Images/ab185953-3-benefits-of-recombinant-antibodies.png)
Anti-EMILIN1 antibody [EPR14678] - N-terminal (ab185953)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab185953 被引用在 2 文献中.
- Deng P et al. The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells. BMC Oral Health 23:203 (2023). PubMed: 37024847
- Rabajdová M et al. Detection of Pathological Changes in the Aorta during Thoracic Aortic Aneurysm Progression on Molecular Level. Dis Markers 2017:9185934 (2017). PubMed: 29158612