Anti-ELYS抗体(ab14431)
Key features and details
- Rabbit polyclonal to ELYS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ELYS抗体
参阅全部 ELYS 一抗 -
描述
兔多克隆抗体to ELYS -
宿主
Rabbit -
特异性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Orangutan
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免疫原
Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa2220 and aa2302 of human ELYS using the numbering given in entry AAN65622.1 (GeneID 25909).
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阳性对照
- HeLa cells nuclear extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 8% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This antibody was purified using an epitope specific to ELYS immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14431于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 272 kDa.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 272 kDa. |
靶标
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功能
Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. -
序列相似性
Belongs to the ELYS family.
Contains 1 A.T hook DNA-binding domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus matrix. Chromosome > centromere > kinetochore. Nucleus > nucleoplasm. Nucleus > nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase. - Information by UniProt
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数据库链接
- Entrez Gene: 25909 Human
- Omim: 610853 Human
- SwissProt: Q8IZA4 Human
- SwissProt: Q8WYP5 Human
- Unigene: 300887 Human
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形式
There are 2 isoforms produced by alternative splicing. -
别名
- ahctf1 antibody
- AT hook containing transcription factor 1 antibody
- DKFZp434N093 antibody
see all
图片
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Western blot - Anti-ELYS antibody (ab14431)All lanes : Anti-ELYS antibody (ab14431) at 0.5 µg/ml
Lane 1 : Cell lysates prepared from Hela cells at 10 µg/ml
Lane 2 : Cell lysates prepared from Hela cells at 20 µg/ml
Predicted band size: 272 kDaab14431 at 0.5mg/ml staining human ELYS by Western blot using chemiluminescence with exposure times of 3-5 minutes. Left lane 10mg HeLa nuclear extract, right lane 20mg.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab14431 被引用在 2 文献中.
- Kuiper EFE et al. The chaperone DNAJB6 surveils FG-nucleoporins and is required for interphase nuclear pore complex biogenesis. Nat Cell Biol 24:1584-1594 (2022). PubMed: 36302971
- Suzuki Y et al. Nuclear formation induced by DNA-conjugated beads in living fertilised mouse egg. Sci Rep 9:8461 (2019). PubMed: 31186495