Anti-ELYS抗体[2318C1a] (ab53540)
![Western blot - Anti-ELYS antibody [2318C1a] (ab53540)](https://www.abcam.cn/ps/products/53/ab53540/Images/ab53540-66419-anti-elys-antibody-2318c1a-western-blot.jpg "Western blot - Anti-ELYS antibody [2318C1a] (ab53540)")
Key features and details
- Mouse monoclonal [2318C1a] to ELYS
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
概述
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产品名称
Anti-ELYS抗体[2318C1a]
参阅全部 ELYS 一抗 -
描述
小鼠单克隆抗体[2318C1a] to ELYS -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Recombinant fragment
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum -
克隆
单克隆 -
克隆编号
2318C1a -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53540于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 253 kDa.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 253 kDa. |
靶标
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功能
Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. -
序列相似性
Belongs to the ELYS family.
Contains 1 A.T hook DNA-binding domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus matrix. Chromosome > centromere > kinetochore. Nucleus > nucleoplasm. Nucleus > nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase. - Information by UniProt
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形式
There are 2 isoforms produced by alternative splicing. -
别名
- ahctf1 antibody
- AT hook containing transcription factor 1 antibody
- DKFZp434N093 antibody
see all
图片
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Western blot - Anti-ELYS antibody [2318C1a] (ab53540)Anti-ELYS antibody [2318C1a] (ab53540) + Immunized recombinant protein
Predicted band size: 253 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as a recombinant protein was used as a test antigen
数据表及文件
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Datasheet download
文献 (1)
ab53540 被引用在 1 文献中.
- Kane M et al. Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Elife 7:N/A (2018). PubMed: 30084827
