重组Anti-Elp4抗体[EPR4200(2)] (ab133687)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4200(2)] to Elp4
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Elp4抗体[EPR4200(2)]
参阅全部 Elp4 一抗 -
描述
兔单克隆抗体[EPR4200(2)] to Elp4 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human Elp4 aa 300-400 (internal sequence). The exact sequence is proprietary.
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阳性对照
- Human stomach tissue; HepG2 cells; 293T, NIH/3T3, Y79, U87-MG and HeLa whole cell lysate (ab150035).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4200(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133687于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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说明 |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
靶标
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功能
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the ELP4 family. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 26610 Human
- Entrez Gene: 77766 Mouse
- Entrez Gene: 691133 Rat
- Omim: 606985 Human
- SwissProt: Q96EB1 Human
- SwissProt: Q9ER73 Mouse
- Unigene: 175534 Human
- Unigene: 33870 Mouse
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别名
- C11orf19 antibody
- dJ68P15A.1 antibody
- elongation protein 4 homolog (S. cerevisiae) antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elp4 antibody [EPR4200(2)] (ab133687)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labelling Elp4 with ab133687 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of HepG2 cells labelling Elp4 with ab133687 at 1/250 dilution.
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All lanes : Anti-Elp4 antibody [EPR4200(2)] (ab133687) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : NIH/3T3 cell lysate
Lane 3 : Y79 cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab133687 被引用在 3 文献中.
- Xu S et al. Epigenetic activation of the elongator complex sensitizes gallbladder cancer to gemcitabine therapy. J Exp Clin Cancer Res 40:373 (2021). PubMed: 34823564
- Li MT et al. ELP3 Acetyltransferase is phosphorylated and regulated by the oncogenic anaplastic lymphoma kinase (ALK). Biochem J 476:2239-2254 (2019). PubMed: 31341009
- Xu S et al. Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer. Nat Commun 10:5492 (2019). PubMed: 31792210