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重组RabMAb

重组Anti-ELOVL5抗体[EPR28824-94] - BSA and Azide free (ab318277)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
  • Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR28824-94] to ELOVL5 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-ELOVL5抗体[EPR28824-94] - BSA and Azide free
    参阅全部 ELOVL5 一抗

  • 描述

    兔单克隆抗体[EPR28824-94] to ELOVL5 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Pmore details
    不适用于: WB

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human breast, Human testis, Jurkat and LNCaP cell pellets, HEK-293T transfected with a ELOVL5 expression vector containing a his tag

  • 常规说明

    ab318277 is the carrier-free version of ab318276

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

相关产品

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab318277于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for WB.

靶标

  • 功能

    Condensing enzyme that catalyzes the synthesis of monounsaturated and of polyunsaturated very long chain fatty acids Acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA.

  • 组织特异性

    Ubiquitous. Highly expressed in the adrenal gland and testis. Weekly expressed in prostate, lung and brain.

  • 序列相似性

    Belongs to the ELO family.

  • 细胞定位

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 3 keto acyl CoA synthase ELOVL5 antibody
      • 3-keto acyl-CoA synthase Elovl5 antibody
      • dJ483K16.1 antibody
      • Elongation of very long chain fatty acids like 5 antibody
      • Elongation of very long chain fatty acids protein 5 antibody
      • ELOV5_HUMAN antibody
      • ELOVL 5 antibody
      • ELOVL family member 5 antibody
      • ELOVL family member 5 elongation of long chain fatty acids antibody
      • ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) antibody
      • ELOVL fatty acid elongase 5 antibody
      • ELOVL2 antibody
      • elovl5 antibody
      • Fatty acid elongase 1 antibody
      • hELO1 antibody
      • Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2 antibody
      • OTTHUMP00000017867 antibody
      • RP3 483K16.1 antibody
      • RP3-483K16.1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

      This data was developed using ab318276, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling ELOVL5 with ab318276 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in human breast.
      The section was incubated with ab318276 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

      This data was developed using ab318276, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ELOVL5 with ab318276 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in human testis.
      The section was incubated with ab318276 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

      This data was developed using ab318276, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cell pellets; (B) LNCaP (human prostate carcinoma epithelial cell) cell pellets; (C) DLD-1 (human colorectal adenocarcinoma epithelial cell) cell pellets; (D) HT-29 (human colorectal adenocarcinoma epithelial cell) cell pellets. tissue labeling ELOVL5 with ab318276 at 1/1000 (0.522 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining in (A) Jurkat and (B) LNCaP cell pellets, while no signal in (C) DLD-1 and (D) HT-29 cell pellets.
      The section was incubated with ab318276 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

      This data was developed using ab318276, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a ELOVL5 expression vector containing a his tag. (B) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling ELOVL5 with ab318276 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on (A) HEK-293T transfected with a ELOVL5 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag.
      The section was incubated with ab318276 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

      This data was developed using ab318276, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ELOVL5 with ab318276 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression tissue: Nearly no staining in human pancreas (PMID: 10970790).
      The section was incubated with ab318276 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

      This data was developed using ab318276, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ELOVL5 with ab318276 at 1/500 (1.044 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression tissue: No staining in human skeletal muscle (PMID: 10970790).
      The section was incubated with ab318276 for 30 mins at room temperature.
      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)
      Anti-ELOVL5 antibody [EPR28824-94] - BSA and Azide free (ab318277)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab318277？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab318277 尚未被引用在任何文献中。

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