Anti-Elongin-C抗体(ab226831)
Key features and details
- Rabbit polyclonal to Elongin-C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Elongin-C抗体
参阅全部 Elongin-C 一抗 -
描述
兔多克隆抗体to Elongin-C -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Cat, Xenopus laevis, Chimpanzee, Rhesus monkey
-
免疫原
Synthetic peptide within Human Elongin-C (internal sequence). The exact sequence is proprietary.
Database link: Q15369 -
阳性对照
- WB: PC-3, Jurkat, Raji and NCI-H929 cell lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226831于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/3000. Predicted molecular weight: 12 kDa.
|
| 说明 |
|---|
|
WB
1/1000 - 1/3000. Predicted molecular weight: 12 kDa. |
靶标
-
功能
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. -
组织特异性
Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3. -
序列相似性
Belongs to the SKP1 family. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 420187 Chicken
- Entrez Gene: 540859 Cow
- Entrez Gene: 6921 Human
- Entrez Gene: 100045866 Mouse
- Entrez Gene: 67923 Mouse
- Entrez Gene: 64525 Rat
- Omim: 600788 Human
- SwissProt: Q2KII4 Cow
see all -
别名
- Elo C antibody
- EloC antibody
- ELOC_HUMAN antibody
see all
图片
-
Western blot - Anti-Elongin-C antibody (ab226831)Anti-Elongin-C antibody (ab226831) at 1/1000 dilution + PC-3 (human prostate adenocarcinoma cell line) cell lysate at 30 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa -
Western blot - Anti-Elongin-C antibody (ab226831)All lanes : Anti-Elongin-C antibody (ab226831) at 1/2000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : NCI-H929 cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 12 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab226831 尚未被引用在任何文献中。