重组Anti-Elongation factor 1-gamma抗体[EPR7200] (ab124994)
![Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)](https://www.abcam.cn/ps/products/124/ab124994/Images/ab124994-139086-anti-elongation-factor-1-gamma-antibody-epr7200-western-blot.jpg "Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7200] to Elongation factor 1-gamma
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Elongation factor 1-gamma抗体[EPR7200]
参阅全部 Elongation factor 1-gamma 一抗 -
描述
兔单克隆抗体[EPR7200] to Elongation factor 1-gamma -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Elongation factor 1-gamma aa 50-150. The exact sequence is proprietary.
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阳性对照
- 293T, HL60, C6, RAW 264.7, PC12, NIH 3T3, HepG2 and HeLa cell lysates; Human pancreatic adenocarcinoma tissue; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 1.31 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7200 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124994于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
|
ICC/IF
1/100 - 1/250. |
靶标
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功能
Probably plays a role in anchoring the complex to other cellular components. -
组织特异性
Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver. -
序列相似性
Contains 1 EF-1-gamma C-terminal domain.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1937 Human
- Entrez Gene: 67160 Mouse
- Entrez Gene: 293725 Rat
- Omim: 130593 Human
- SwissProt: P26641 Human
- SwissProt: Q9D8N0 Mouse
- SwissProt: Q68FR6 Rat
- Unigene: 144835 Human
see all -
别名
- 2610301D06Rik antibody
- AA407312 antibody
- eEF 1B gamma antibody
see all
图片
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Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)All lanes : Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HL-60 cell lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW 264.7 cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 50 kDa -
![Flow Cytometry (Intracellular) - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)](https://www.abcam.cn/ps/products/124/ab124994/Images/ab124994-215917-anti-elongation-factor-1-gamma-antibody-epr7200-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)](https://www.abcam.cn/ps/products/124/ab124994/Images/ab124994-139085-anti-elongation-factor-1-gamma-antibody-epr7200-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)ab124994 at 1/250 dilution staining Elongation factor 1-gamma in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)](https://www.abcam.cn/ps/products/124/ab124994/Images/ab124994-139084-anti-elongation-factor-1-gamma-antibody-epr7200-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)ab124994 at 1/100 dilution staining Elongation factor 1-gamma in HeLa cells by Immunofluorescence.
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![OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)](https://www.abcam.cn/ps/products/124/ab124994/Images/ab124994-139083-anti-elongation-factor-1-gamma-antibody-epr7200.jpg)
OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994) -
![Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)](https://www.abcam.cn/ps/products/124/ab124994/Images/ab124994-6-benefits-of-recombinant-antibodies.png)
Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124994 被引用在 2 文献中.
- Sammaibashi S et al. Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins. Front Microbiol 9:1446 (2018). PubMed: 30008712
- Milone MR et al. Proteomic analysis of zoledronic-acid resistant prostate cancer cells unveils novel pathways characterizing an invasive phenotype. Oncotarget 6:5324-41 (2015). WB . PubMed: 25481874