Anti-Elongation factor 1-gamma抗体(ab72368)
Key features and details
- Rabbit polyclonal to Elongation factor 1-gamma
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Elongation factor 1-gamma抗体
参阅全部 Elongation factor 1-gamma 一抗 -
描述
兔多克隆抗体to Elongation factor 1-gamma -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IP, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide corresponding to Human Elongation factor 1-gamma aa 200-300.
Database link: P26641 -
阳性对照
- WB: Whole cell lysate from Hela, 293T, NIH3T3. IHC-P: Human colon carcinoma and testis tissue. ICC/IF: Hela whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72368于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/2000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Probably plays a role in anchoring the complex to other cellular components. -
组织特异性
Highly expressed in pancreatic tumor tissue and to a lesser extent in normal kidney, intestine, pancreas, stomach, lung, brain, spleen and liver. -
序列相似性
Contains 1 EF-1-gamma C-terminal domain.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1937 Human
- Entrez Gene: 67160 Mouse
- Omim: 130593 Human
- SwissProt: P26641 Human
- SwissProt: Q9D8N0 Mouse
- Unigene: 144835 Human
- Unigene: 444467 Human
- Unigene: 247762 Mouse
see all -
别名
- 2610301D06Rik antibody
- AA407312 antibody
- eEF 1B gamma antibody
see all
图片
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Western blot - Anti-Elongation factor 1-gamma antibody (ab72368)All lanes : Anti-Elongation factor 1-gamma antibody (ab72368) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody (ab72368)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Elongation factor 1-gamma with ab72368 at 1/200 (1 µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Elongation factor 1-gamma antibody (ab72368)Immunoprecipitation/ Western Blot of Elongation factor 1-gamma. Lane 1: ab72368 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72368 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody (ab72368)IHC image of ab72368 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Elongation factor 1-gamma antibody (ab72368)ICC image of ab72368 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72368, 1 µg/mL) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab72368 被引用在 5 文献中.
- Pellegrini L et al. Proteomic analysis reveals co-ordinated alterations in protein synthesis and degradation pathways in LRRK2 knockout mice. Hum Mol Genet 27:3257-3271 (2018). PubMed: 29917075
- Ayre DC et al. CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo. Sci Rep 7:8642 (2017). PubMed: 28819186
- Yang J et al. Site-specific mapping and quantification of protein S-sulphenylation in cells. Nat Commun 5:4776 (2014). Human . PubMed: 25175731
- Bellé R et al. Identification of a new isoform of eEF2 whose phosphorylation is required for completion of cell division in sea urchin embryos. Dev Biol 350:476-83 (2011). WB ; Sea urchin . PubMed: 21167828
- Peng S et al. Genome-wide studies reveal that Lin28 enhances the translation of genes important for growth and survival of human embryonic stem cells. Stem Cells 29:496-504 (2011). PubMed: 21425412