Anti-ELMO2抗体(ab2240)
Key features and details
- Goat polyclonal to ELMO2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ELMO2抗体 -
描述
山羊多克隆抗体to ELMO2 -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig
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免疫原
Synthetic peptide: PKEPSSYDFVYHYG, corresponding to C terminal amino acids 707-720 of Human ELMO2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 1 - 3 µg/ml.
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
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IHC-P
Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 3 µg/ml. 1 hour primary incubation is recommended for this product. |
靶标
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功能
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. -
组织特异性
Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. -
序列相似性
Contains 1 ELMO domain.
Contains 1 PH domain. -
细胞定位
Cytoplasm. Cytoplasm > cytosol. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 508361 Cow
- Entrez Gene: 63916 Human
- Entrez Gene: 140579 Mouse
- Entrez Gene: 362271 Rat
- Omim: 606421 Human
- SwissProt: A4FUD6 Cow
- SwissProt: Q96JJ3 Human
- SwissProt: Q8BHL5 Mouse
see all -
别名
- CED 12 antibody
- Ced 12 homolog 2 antibody
- CED 12 homolog A antibody
see all
图片
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Western blot - Anti-ELMO2 antibody (ab2240)Ab2240 staining (2
µ g/ml) of Human Brain lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Ab2240 staining (2µg/ml) of Human Brain lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab2240 被引用在 7 文献中.
- Wang Y et al. ELMO2 association with Gai2 regulates pancreatic cancer cell chemotaxis and metastasis. PeerJ 8:e8910 (2020). PubMed: 32292657
- Hetmanski JHR et al. Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration. Dev Cell 51:460-475.e10 (2019). PubMed: 31607653
- Toret CP et al. A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion. J Cell Biol 204:265-79 (2014). ICC/IF, WB ; Drosophila melanogaster . PubMed: 24446484
- Toret CP et al. An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion. J Cell Biol 207:577-87 (2014). PubMed: 25452388
- Harada K et al. Ephexin4 and EphA2 mediate resistance to anoikis through RhoG and phosphatidylinositol 3-kinase. Exp Cell Res 317:1701-13 (2011). WB ; Human . PubMed: 21621533
- Hiramoto-Yamaki N et al. Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism. J Cell Biol 190:461-77 (2010). WB ; Human . PubMed: 20679435
- Gekonge BN et al. Signal transduction induced by apoptotic cells inhibits HIV transcription in monocytes/macrophages. J Leukoc Biol 80:953-60 (2006). WB . PubMed: 16885500
