AB2240

Anti-ELMO2抗体

Name: ELMO2抗体 (ab2240)| Abcam中文官网
Brand: Abcam Limited
SKU: AB2240
Price: 4606 CNY
Availability: InStock

Anti-ELMO2 antibody

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(7 Publications)

Goat Polyclonal ELMO2 antibody. Suitable for Flow Cyt, IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ELMO2 aa 700 to C-terminus.

查看别名

CED12A, KIAA1834, ELMO2, Engulfment and cell motility protein 2, Protein ced-12 homolog A, hCed-12A

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).

Supplier Data

Western blot - Anti-ELMO2 antibody (AB2240)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-ELMO2 antibody (ab2240) at 0.5 µg

Lane 1:

Jurkat cell lysate at 35 µg/mL

Lane 2:

HEK293 cell lysate at 35 µg/mL

Lane 3:

RAW264.7 cell lysate at 35 µg/mL

Lane 4:

KNRK cell lysate at 35 µg/mL

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Supplier Data

Western blot - Anti-ELMO2 antibody (AB2240)

All lanes:

Western blot - Anti-ELMO2 antibody (ab2240) at 0.5 µg/mL

Lane 2:

Human cerebellum tissue lysate at 35 µg

Lane 2:

Rat brain tissue lysate at 35 µg

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Flow Cytometry - Anti-ELMO2 antibody (AB2240)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ELMO2 antibody (AB2240)

Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, Flow Cyt, WB, IHC-P

applications

免疫原

Synthetic Peptide within Human ELMO2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96JJ3

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3-6 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.05% Tris

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ELMO2 also known as Engulfment and Cell Motility Protein 2 mechanically functions as an important regulator of cell migration and phagocytosis. It weighs approximately 82 kDa. This protein interacts extensively with the cytoplasmic tail of transmembrane receptors aiding in intracellular signaling cascades. ELMO2 is expressed in various tissues with notable levels in the brain kidney and spleen indicating its wide biological roles.

Biological function summary

ELMO2 plays a significant role in the regulation of the actin cytoskeleton which is important in cell motility and shape. It forms part of the ELMO/EPS8/SOS1 complex where it facilitates actin remodeling and cell surface receptor signaling. The protein acts as a guanine nucleotide exchange factor (GEF) guiding the activation of small GTPases like Rac1 essential for cytoskeletal reorganization and cellular responses to external stimuli.

Pathways

ELMO2 engages importantly in the PI3K/AKT signaling pathway and the phagocytosis regulatory pathway. It collaborates with the proteins such as DOCK180 functioning together in the phagocytosis of apoptotic cells. This association ensures the efficient clearance of dying cells maintaining tissue homeostasis and immune system balance.

ELMO2 connects directly to several cancer types and neurodegenerative diseases. Its dysregulation is often observed in certain cancers such as breast cancer where it influences metastasis via alterations in cell migration. ELMO2 is also linked to Alzheimer's disease interacting with proteins like amyloid precursor protein (APP) suggesting a role in the pathological changes seen in neuronal cell environments.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

See full target information ELMO2

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 8:e8910 PubMed32292657

2020

ELMO2 association with Gi2 regulates pancreatic cancer cell chemotaxis and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yecheng Wang,Hongyan Li,Fei Li

Developmental cell 51:460-475.e10 PubMed31607653

2019

Membrane Tension Orchestrates Rear Retraction in Matrix-Directed Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph H R Hetmanski,Henry de Belly,Ignacio Busnelli,Thomas Waring,Roshna V Nair,Vanesa Sokleva,Oana Dobre,Angus Cameron,Nils Gauthier,Christophe Lamaze,Joe Swift,Aránzazu Del Campo,Tobias Starborg,Tobias Zech,Jacky G Goetz,Ewa K Paluch,Jean-Marc Schwartz,Patrick T Caswell

The Journal of cell biology 207:577-87 PubMed25452388

2014

An Elmo-Dock complex locally controls Rho GTPases and actin remodeling during cadherin-mediated adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher P Toret,Caitlin Collins,W James Nelson

The Journal of cell biology 204:265-79 PubMed24446484

2014

A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell-cell adhesion.

Applications

WB, ICC/IF

Species

Drosophila melanogaster, Drosophila melanogaster

Christopher P Toret,Michael V D'Ambrosio,Ronald D Vale,Michael A Simon,W James Nelson

Experimental cell research 317:1701-13 PubMed21621533

2011

Ephexin4 and EphA2 mediate resistance to anoikis through RhoG and phosphatidylinositol 3-kinase.

Applications

Species

Human

Kohei Harada,Nao Hiramoto-Yamaki,Manabu Negishi,Hironori Katoh

The Journal of cell biology 190:461-77 PubMed20679435

2010

Ephexin4 and EphA2 mediate cell migration through a RhoG-dependent mechanism.

Applications

Species

Human

Nao Hiramoto-Yamaki,Shingo Takeuchi,Shuhei Ueda,Kohei Harada,Satoshi Fujimoto,Manabu Negishi,Hironori Katoh

Journal of leukocyte biology 80:953-60 PubMed16885500

2006

Signal transduction induced by apoptotic cells inhibits HIV transcription in monocytes/macrophages.

Applications

Species

Unspecified reactive species

Bethsebah N Gekonge,Gillian Schiralli,Robert A Schlegel,Andrew J Henderson

View all publications

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Anti-ELMO2抗体

Anti-ELMO2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Immunocytochemistry/ Immunofluorescence - Anti-ELMO2 antibody (AB2240)

Western blot - Anti-ELMO2 antibody (AB2240)

Western blot - Anti-ELMO2 antibody (AB2240)

Flow Cytometry - Anti-ELMO2 antibody (AB2240)