Anti-ELK1 (phospho T417)抗体(ab28817)
Key features and details
- Rabbit polyclonal to ELK1 (phospho T417)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ELK1 (phospho T417)抗体
参阅全部 ELK1 一抗 -
描述
兔多克隆抗体to ELK1 (phospho T417) -
宿主
Rabbit -
特异性
ab28817 recognises Phospho-ELK-1(Thr417). -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human ELK1 (phospho T417).
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阳性对照
- Breast carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28817于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. -
组织特异性
Lung and testis. -
序列相似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain. -
翻译后修饰
Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 2002 Human
- Entrez Gene: 13712 Mouse
- Omim: 311040 Human
- SwissProt: P19419 Human
- SwissProt: P41969 Mouse
- Unigene: 181128 Human
- Unigene: 405823 Mouse
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别名
- ELK 1 antibody
- Elk1 antibody
- ELK1 member of ETS oncogene family antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ELK1 (phospho T417) antibody (ab28817)Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Phospho-ELK-1(Thr417) Antibody (ab28817); Right: The same antibody preincubated with synthesized phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab28817 尚未被引用在任何文献中。