Anti-Elastin抗体[BA-4] (ab9519)
Key features and details
- Mouse monoclonal [BA-4] to Elastin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Elastin抗体[BA-4]
参阅全部 Elastin 一抗 -
描述
小鼠单克隆抗体[BA-4] to Elastin -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Human
预测可用于: Sheep, Goat, Guinea pig, Cat, Dog, Pig -
免疫原
The details of the immunogen for this antibody are not available.
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表位
BA-4 is specific for a chemotactically active epitope composed of valine, glycine, alanine, and proline in a molar ratio of approximately 2:2:1:1 (PMID 2429696). -
阳性对照
- WB: Human skin tissue lysate. IHC-P: FFPE normal Human Duodenum tissue sections IHC-Fr: Human normal skin
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
Primary antibody说明
Elastin is an important polymeric protein of connective tissue that imparts elasticity to vertebrate elastic tissues. -
克隆
单克隆 -
克隆编号
BA-4 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.51 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
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IHC-P | (6) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr | (2) |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 0.51 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
Use a concentration of 1 µg/ml. |
靶标
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功能
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. -
组织特异性
Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin. -
疾病相关
Defects in ELN are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Cutis laxa is a rare connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. The skin changes are often accompanied by extracutaneous manifestations, including pulmonary emphysema, bladder diverticula, pulmonary artery stenosis and pyloric stenosis.
Defects in ELN are the cause of supravalvular aortic stenosis (SVAS) [MIM:185500]. SVAS is a congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
Note=ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
序列相似性
Belongs to the elastin family. -
翻译后修饰
Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
细胞定位
Secreted > extracellular space > extracellular matrix. Extracellular matrix of elastic fibers. - Information by UniProt
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数据库链接
- Entrez Gene: 2006 Human
- Omim: 130160 Human
- SwissProt: P15502 Human
- Unigene: 647061 Human
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别名
- Elastin antibody
- ELN antibody
- ELN_HUMAN antibody
see all
图片
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IHC image of Elastin staining in a section of formalin-fixed paraffin-embedded normal human normal duodenum* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab9519, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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IHC image of ab9519 staining in 10% formaldehyde fixed frozen tissue section of normal human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1h at room temperature. The section was then incubated with ab9519 (1μg/ml) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab9519.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
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ab9519 staining pig small intestine tissue sections by IHC-P. Sections were fixed with Bouins and subjected to an enzymatic antigen retreival step. The primary antibody was diluted 1/200 in a commercially available antibody diluent (replacing the blocking step) and incubated with the sample for 1 hour at 20°C. A HRP conjugated goat anti-mouse antibody was used as the secondary.
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Anti-Elastin antibody [BA-4] (ab9519) at 0.51 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (32)
ab9519 被引用在 32 文献中.
- Matias GSS et al. Ionic Detergent Under Pressure-Vacuum as an Innovative Strategy to Generate Canine Tracheal Scaffold for Organ Engineering. Cells Tissues Organs 212:535-545 (2023). PubMed: 35640555
- Daryabari SS et al. Whole-organ decellularization of the human uterus and in vivo application of the bio-scaffolds in animal models. J Assist Reprod Genet 39:1237-1247 (2022). PubMed: 35513746
- Ramakrishnan R et al. Extracellular matrix-based combination scaffold for guided regeneration of large-area full-thickness rabbit burn wounds upon a single application. J Biomed Mater Res B Appl Biomater 110:848-861 (2022). PubMed: 34773682
- Li J et al. Cadherin-11 promotes the mechanical strength of engineered elastic cartilage by enhancing extracellular matrix synthesis and microstructure. J Tissue Eng Regen Med 16:188-199 (2022). PubMed: 34837334
- Cho KH et al. Optic nerve-associated connective tissue structures revisited: A histological study using human fetuses and adult cadavers. Anat Rec (Hoboken) 305:3516-3531 (2022). PubMed: 35358354