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Anti-EKLF / KLF1抗体[1B6A3] (ab175372)

Submit a review Submit a question References (2)
Western blot - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
  • Flow Cytometry - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

Key features and details

  • Mouse monoclonal [1B6A3] to EKLF / KLF1
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-EKLF / KLF1抗体[1B6A3]

  • 描述

    小鼠单克隆抗体[1B6A3] to EKLF / KLF1

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, Flow Cyt, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment corresponding to Human EKLF/ KLF1 aa 208-362. Expressed in E. Coli.
    Sequence:

    PAPQYQGHFQLFRGLQGPAPGPATSPSFLSCLGPGTVGTGLGGTAEDPGV IAETAPSKRGRRSWARKRQAAHTCAHPGCGKSYTKSSHLKAHLRTHTGEK PYACTWEGCGWRFARSDELTRHYRKHTGQRPFRCQLCPRAFSRSDHLALH MKRHL


    Database link: Q13351
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Human EKLF / KLF1 recombinant protein; HeLa cells; Human cervical and rectum cancer tissues

  • 常规说明

    This product was changed from ascites to supernatant. Lot no’s high than GR252532-8 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab175372于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/2000. Predicted molecular weight: 38 kDa.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.
说明
WB
1/500 - 1/2000. Predicted molecular weight: 38 kDa.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P
1/200 - 1/1000.

靶标

  • 功能

    Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis. Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.

  • 组织特异性

    Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.

  • 疾病相关

    Defects in KLF1 are the cause of congenital dyserythropoietic anemia type 4 (CDA4) [MIM:613673]. It is a blood disorder characterized by ineffective erythropoiesis and hemolysis resulting in anemia. Circulating erythroblasts and erythroblasts in the bone marrow show various morphologic abnormalities. Affected individuals with CDA4 also have increased levels of fetal hemoglobin.

  • 序列相似性

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.

  • 翻译后修饰

    Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.
    Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.
    Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.

  • 细胞定位

    Nucleus. Colocalizes with SUMO1 in nuclear speckles.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CDAN4 antibody
      • EKLF antibody
      • EKLF PEN antibody
      • Erythroid krueppel like transcription factor (EKLF) (Erythroid transcription factor) antibody
      • Erythroid krueppel-like transcription factor antibody
      • Erythroid Kruppel like factor antibody
      • Erythroid specific transcription factor EKLF antibody
      • HBFQTL6 antibody
      • Human erythroid-specific transcription factor EKLF mRNA complete cds antibody
      • INLU antibody
      • Klf1 antibody
      • KLF1_HUMAN antibody
      • Krueppel-like factor 1 antibody
      • Kruppel like factor 1 (erythroid) antibody
      see all

    图片

    • Western blot - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
      Western blot - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
      Anti-EKLF / KLF1 antibody [1B6A3] (ab175372) at 1/500 dilution + Human EKLF / KLF1 recombinant protein

      Predicted band size: 38 kDa



      Expected MW is 42.6 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

      Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling EKLF / KLF1 using ab175372 at 1/200 dilution, followed by DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

      Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling EKLF / KLF1 using ab175372 at 1/200 dilution, followed by DAB staining.

    • Flow Cytometry - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)
      Flow Cytometry - Anti-EKLF / KLF1 antibody [1B6A3] (ab175372)

      Flow cytometric analysis of HeLa cells labeling EKLF / KLF1 using ab175372 at 1/200 dilution (green) and negative control (red).

    文献 (2)

    发表研究结果有使用 ab175372？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab175372 被引用在 2 文献中.

    • Liang L  et al. Deubiquitylase USP7 regulates human terminal erythroid differentiation by stabilizing GATA1. Haematologica N/A:N/A (2019). PubMed: 30872372
    • de Vasconcellos JF  et al. IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts. Proc Natl Acad Sci U S A 114:E5664-E5672 (2017). WB ; Human . PubMed: 28652347

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