Anti-eIF4EBP1 (phospho T46)抗体(ab27792)
Key features and details
- Rabbit polyclonal to eIF4EBP1 (phospho T46)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-eIF4EBP1 (phospho T46)抗体
参阅全部 eIF4EBP1 一抗 -
描述
兔多克隆抗体to eIF4EBP1 (phospho T46) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human eIF4EBP1 (phospho T46).
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阳性对照
- WB: PI3K inhibitor LY2904002 and PDGF treated and untreated NIH3T3 cell lysate. ICC/IF: 70% confluent log phase HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Ab27792 was purified from rabbit serum by sequential epitope specific chromatography. The antibody was negatively preadsorbed using a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated eIF4EBP1. The final product is generated by affinity chromatography using a eIF4EBP1 derived peptide that is phosphorylated at threonine 46. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27792于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 13 kDa.
|
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| ICC/IF |
1/250.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 13 kDa. |
|
ICC/IF
1/250. |
靶标
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功能
Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. -
序列相似性
Belongs to the eIF4E-binding protein family. -
翻译后修饰
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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数据库链接
- Entrez Gene: 1978 Human
- Entrez Gene: 13685 Mouse
- Omim: 602223 Human
- SwissProt: Q13541 Human
- SwissProt: Q60876 Mouse
- Unigene: 411641 Human
- Unigene: 6700 Mouse
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别名
- 4E-BP1 antibody
- 4EBP1 antibody
- 4EBP1_HUMAN antibody
see all
图片
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Western blot - Anti-eIF4EBP1 (phospho T46) antibody (ab27792)All lanes : Anti-eIF4EBP1 (phospho T46) antibody (ab27792) at 1/1000 dilution (diluted in 3% BSA TBST buffer, incubated for two
hours at room temperature.)
Lane 1 : PDGF treated NIH3T3 cell lysate
Lane 2 : PI3K inhibitor LY2904002 treated NIH3T3 cell lysate
Lane 3 : PI3K inhibitor LY2904002 and PDGF treated NIH3T3 cell lysate
Secondary
All lanes : goat F(ab)2 anti rabbit IgG
HRP conjugate
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
The figure shows that ab27792 can detect phosphorylated eIF4EBP1 in PDGF treated NIH3T3 cells (lane 1), but does not detect the unphosphorylated form (lanes 2 and 3: controls). Blocking step: 3% BSA TBST buffer for one hour at room temperature. -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4EBP1 (phospho T46) antibody (ab27792)Immunofluorescence analysis of eIF4EBP1 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with 4EBP1 (pT46) Rabbit Polyclonal Antibody (ab27792) at 1/250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1/300). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab27792 被引用在 4 文献中.
- Zhang LX et al. TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway. Cancer Sci 114:855-869 (2023). PubMed: 36403197
- Wang H et al. mTOR Modulates the Endoplasmic Reticulum Stress-Induced CD4+ T Cell Apoptosis Mediated by ROS in Septic Immunosuppression. Mediators Inflamm 2022:6077570 (2022). PubMed: 35915740
- Liu S et al. Circ_0119872 promotes uveal melanoma development by regulating the miR-622/G3BP1 axis and downstream signalling pathways. J Exp Clin Cancer Res 40:66 (2021). PubMed: 33579337
- Lauria F et al. SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Nat Cell Biol 22:1239-1251 (2020). PubMed: 32958857