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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation

Anti-eIF4EBP1抗体(ab2606)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (28)

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Western blot - Anti-eIF4EBP1 antibody (ab2606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
  • Western blot - Anti-eIF4EBP1 antibody (ab2606)

Key features and details

  • Rabbit polyclonal to eIF4EBP1
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-eIF4EBP1 antibody [Y329] (ab32024)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-eIF4EBP1抗体
    参阅全部 eIF4EBP1 一抗
  • 描述

    兔多克隆抗体to eIF4EBP1
  • 宿主

    Rabbit
  • 特异性

    This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.
  • 经测试应用

    适用于: IHC-P, WBmore details
  • 种属反应性

    与反应: Rat, Human
    预测可用于: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯化说明

    Affinity purified using the immunising peptideimmobilized on solid support.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human eIF4EBP1 protein (ab60007)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2606于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P (1)
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (2)
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
说明
IHC-P
1/5000 - 1/20000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.

靶标

  • 功能

    Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
  • 序列相似性

    Belongs to the eIF4E-binding protein family.
  • 翻译后修饰

    Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70 is regulated by mTORC1. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Target information above from: UniProt accession Q13541 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1978 Human
    • Entrez Gene: 116636 Rat
    • Omim: 602223 Human
    • SwissProt: Q13541 Human
    • SwissProt: Q62622 Rat
    • Unigene: 411641 Human
    • Unigene: 11161 Rat
    • 别名

      • 4E-BP1 antibody
      • 4EBP1 antibody
      • 4EBP1_HUMAN antibody
      • BP 1 antibody
      • eIF4E binding protein 1 antibody
      • eIF4E-binding protein 1 antibody
      • Eif4ebp1 antibody
      • Eukaryotic translation initiation factor 4E-binding protein 1 antibody
      • PHAS-I antibody
      • PHASI antibody
      • Phosphorylated heat- and acid-stable protein regulated by insulin 1 antibody
      see all

    图片

    • Western blot - Anti-eIF4EBP1 antibody (ab2606)
      Western blot - Anti-eIF4EBP1 antibody (ab2606)

      Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of  eIF-4EBP1 in 100µg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
      after exposure to high (lane 1) and low (lane 2) concentrations of leucine. Ab2606 at a 1:4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100µg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (ab2606)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection: DAB.
    • Western blot - Anti-eIF4EBP1 antibody (ab2606)
      Western blot - Anti-eIF4EBP1 antibody (ab2606)
      All lanes : Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution

      Lane 1 : Recombinant Human eIF4EBP1 protein (ab60007) at 0.01 µg
      Lane 2 : Recombinant Human eIF4EBP1 protein (ab60007) at 0.001 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 13 kDa


      Exposure time: 10 seconds

    实验方案

    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (28)

    发表研究结果有使用 ab2606?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2606 被引用在 28 文献中.

    • Tian Y  et al. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol 235:442-453 (2020). PubMed: 31332784
    • Lauria F  et al. SMN-primed ribosomes modulate the translation of transcripts related to spinal muscular atrophy. Nat Cell Biol 22:1239-1251 (2020). PubMed: 32958857
    • Morales-Garcia V  et al. The high-risk HPV E6 proteins modify the activity of the eIF4E protein via the MEK/ERK and AKT/PKB pathways. FEBS Open Bio 10:2541-2552 (2020). PubMed: 32981220
    • Yao R  et al. Pathogenic effects of inhibition of mTORC1/STAT3 axis facilitates Staphylococcus aureus-induced pyroptosis in human macrophages. Cell Commun Signal 18:187 (2020). PubMed: 33256738
    • Guo GF  et al. Predictive and prognostic implications of 4E-BP1, Beclin-1, and LC3 for cetuximab treatment combined with chemotherapy in advanced colorectal cancer with wild-type KRAS: Analysis from real-world data. World J Gastroenterol 25:1840-1853 (2019). PubMed: 31057298
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-eIF4EBP1 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: CC1 Antigen Retrieval Buffer
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Rodent block M as blocking agent for 12 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 07 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-eIF4EBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Vascular smooth muscle cell)
    Permeabilization
    Yes - NP40
    Specification
    Vascular smooth muscle cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 20 2018

    Western blot abreview for Anti-eIF4EBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount
    10 µg
    Specification
    NIH 3T3 cells
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 18 2009

    Western blot abreview for Anti-eIF4EBP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HT1080 cell line)
    Loading amount
    10 µg
    Specification
    HT1080 cell line
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 18 2009

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