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AB2606

Anti-eIF4EBP1 抗体

Anti-eIF4EBP1 antibody

5

(4 Reviews)

|

(37 Publications)

Rabbit Polyclonal eIF4EBP1 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 37 publications. Immunogen corresponding to Synthetic Peptide within Human EIF4EBP1.

查看别名

Eukaryotic translation initiation factor 4E-binding protein 1, 4E-BP1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, EIF4EBP1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (AB2606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF4EBP1 antibody (AB2606)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling eIF4EBP1 with ab2606 at 1/20000 (0.05µg/ml). Detection : DAB.

Western blot - Anti-eIF4EBP1 antibody (AB2606)
  • WB

Unknown

Western blot - Anti-eIF4EBP1 antibody (AB2606)

ab2606 at a 1 : 4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100μg of RIN-m5F (Rat insulinoma cell line) whole cell lysate
after exposure to high (lane 1) and low (lane 2) concentrations of leucine. ab2606 at a 1 : 4000 dilution staining alpha, beta and gamma isoforms of eIF-4EBP1 in 100μg of RINm5F cell lysate after exposure to high (lane 1) and low (lane 2) concentrations of leucine.

All lanes:

Western blot - Anti-eIF4EBP1 antibody (ab2606)

Predicted band size: 13 kDa

false

Western blot - Anti-eIF4EBP1 antibody (AB2606)
  • WB

Unknown

Western blot - Anti-eIF4EBP1 antibody (AB2606)

All lanes:

Western blot - Anti-eIF4EBP1 antibody (ab2606) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human eIF4EBP1 protein (<a href='/products/proteins-peptides/recombinant-human-eif4ebp1-protein-ab60007'>ab60007</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant Human eIF4EBP1 protein (<a href='/products/proteins-peptides/recombinant-human-eif4ebp1-protein-ab60007'>ab60007</a>) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 13 kDa

true

Exposure time: 10s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Rat, Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human EIF4EBP1. The exact immunogen used to generate this antibody is proprietary information.

Q13541

特异性

This antibody detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/20000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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您是否考虑以下替代产品？

AB32024

Anti-eIF4EBP1 antibody [Y329]

0
0 Reviews
查看产品
为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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性能和储存信息

形式
Liquid
纯化说明
Affinity purified using the immunising peptideimmobilized on solid support.
存储溶液
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EIF4EBP1 also known as 4EBP1 or p4EBP1 is an important protein that acts as a translational repressor by binding to eIF4E therefore inhibiting cap-dependent translation. The molecular weight of 4EBP1 is approximately 12 kDa. This protein is ubiquitously expressed in various tissues indicating its widespread involvement in cellular functions. eIF4EBP1 can be detected and quantified using techniques like the eIF4EBP1 ELISA making it a common target in research for its role in translation regulation.
Biological function summary

EIF4EBP1 plays an important role in regulating cell growth and proliferation by modulating protein synthesis. It is a part of the eIF4F complex which is responsible for the initiation of mRNA translation. When hypophosphorylated eIF4EBP1 binds tightly to eIF4E and prevents the assembly of the active eIF4F complex leading to reduced translation initiation of mRNAs involved in growth and survival. This regulation is important in conditions where cells need to adapt to metabolic stress or external signals.

Pathways

The mammalian target of rapamycin (mTOR) pathway regulates eIF4EBP1 through phosphorylation. In response to growth signals mTOR phosphorylates eIF4EBP1 causing the release of eIF4E and allowing mRNA translation to proceed. This interaction links eIF4EBP1 to the PI3K/AKT/mTOR signaling pathway which influences cell cycle progression and survival. Related proteins in this pathway include ribosomal protein S6 kinase (S6K1) which is also phosphorylated by mTOR to promote protein synthesis.

Deregulation of eIF4EBP1 has been implicated in cancer and neurological disorders. In cancer hyperactive mTOR signaling can lead to excessive phosphorylation of 4EBP1 decreasing its ability to inhibit eIF4E and enhancing translation of oncogenic proteins. In neurological disorders imbalanced eIF4EBP1 activity can disrupt synaptic plasticity and memory formation. Proteins such as p70S6K which are also part of the mTOR pathway share connections with eIF4EBP1 in these pathological conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex : hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
See full target information EIF4EBP1
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文献 (37)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 21:e3002355 PubMed37943958

2023

Nonsense mutation suppression is enhanced by targeting different stages of the protein synthesis process.

Applications

Unspecified application

Species

Unspecified reactive species

Amnon Wittenstein,Michal Caspi,Ido Rippin,Orna Elroy-Stein,Hagit Eldar-Finkelman,Sven Thoms,Rina Rosin-Arbesfeld

Frontiers in nutrition 10:1098715 PubMed36969813

2023

Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yingao Qi,Tenghui Zheng,Xinghong Liu,Siwang Yang,Qihui Li,Jiayuan Shao,Xiangfang Zeng,Wutai Guan,Shihai Zhang

Journal of animal science and technology 64:922-936 PubMed36287778

2022

Effect of serotonin on the cell viability of the bovine mammary alveolar cell-T (MAC-T) cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Xusheng Dong,Chen Liu,Jialin Miao,Xueyan Lin,Yun Wang,Zhonghua Wang,Qiuling Hou

Autophagy 19:1087-1099 PubMed35998113

2022

NPPA/atrial natriuretic peptide is an extracellular modulator of autophagy in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Maurizio Forte,Simona Marchitti,Flavio Di Nonno,Rosita Stanzione,Leonardo Schirone,Maria Cotugno,Franca Bianchi,Sonia Schiavon,Salvatore Raffa,Danilo Ranieri,Salvatore Fioriniello,Floriana Della Ragione,Maria Rosaria Torrisi,Roberto Carnevale,Valentina Valenti,Francesco Versaci,Giacomo Frati,Carmine Vecchione,Massimo Volpe,Speranza Rubattu,Sebastiano Sciarretta

Clinical, cosmetic and investigational dermatology 15:643-651 PubMed35444442

2022

Enhanced Activation of mTOR Signaling Pathway Was Found in the Hypertrophic and Nodular Lesions of Port Wine Stains.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Nan Xu,Qian Wang,Min Wang,Yuan Xu,Si-Ming Yuan

Oncology letters 23:70 PubMed35069879

2022

CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1-mediated Warburg effect.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Yang,Yanchun Wang,Chunhui Tao,Yunhai Li,Shan Cao,Xiqian Yang

Frontiers in veterinary science 8:756375 PubMed34869729

2021

Intracellular Infection Decreases Milk Protein Synthesis by Preventing Amino Acid Uptake in Bovine Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhao Chen,Yuze Ma,Qiang Ji,Xiaoru Yang,Xue Feng,Ruiyuan Yao,Xiaoou Cheng,Tingting Li,Yanfeng Wang,Zhigang Wang

Experimental & molecular medicine 53:1207-1217 PubMed34385569

2021

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Zhao,Bingyan Li,Yongxia Ren,Tiansong Liang,Juan Wang,Suna Zhai,Xiqian Zhang,Pengcheng Zhou,Xiangxian Zhang,Yuanyuan Pan,Fangfang Gao,Sulan Zhang,Liming Li,Yongqiang Yang,Xiaoyu Deng,Xiaole Li,Linhui Chen,Daoke Yang,Yingjuan Zheng

Animals : an open access journal from MDPI 11: PubMed33375218

2020

Fat Encapsulation Reduces Diarrhea in Piglets Partially by Repairing the Intestinal Barrier and Improving Fatty Acid Transport.

Applications

Unspecified application

Species

Unspecified reactive species

Min Tian,Jiaming Chen,Zhihui Wu,Hanqing Song,Fei Yang,Chang Cui,Fang Chen,Shihai Zhang,Wutai Guan

Cell communication and signaling : CCS 18:187 PubMed33256738

2020

Pathogenic effects of inhibition of mTORC1/STAT3 axis facilitates Staphylococcus aureus-induced pyroptosis in human macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiyuan Yao,Yuhao Chen,Huifang Hao,Zhixin Guo,Xiaoou Cheng,Yuze Ma,Qiang Ji,Xiaoru Yang,Yanfeng Wang,Xihe Li,Zhigang Wang
View all publications
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