Anti-eIF4E抗体(ab72116)
Key features and details
- Rabbit polyclonal to eIF4E
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-eIF4E抗体
参阅全部 eIF4E 一抗 -
描述
兔多克隆抗体to eIF4E -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Elephant
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免疫原
Synthetic peptide corresponding to a region between residue 1 and 50 of human eIF4E (NP_001959.1).
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阳性对照
- HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72116 was affinity purified using an epitope specific to eIF4E immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72116于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| WB |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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| 说明 |
|---|
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IP
Use at 2-5 µg/mg of lysate. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
靶标
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功能
Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. -
序列相似性
Belongs to the eukaryotic initiation factor 4E family. -
翻译后修饰
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. - Information by UniProt
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数据库链接
- Entrez Gene: 281751 Cow
- Entrez Gene: 1977 Human
- Entrez Gene: 13684 Mouse
- Omim: 133440 Human
- SwissProt: Q9N0T5 Cow
- SwissProt: P06730 Human
- SwissProt: P63073 Mouse
- SwissProt: P29338 Rabbit
see all -
别名
- AUTS19 antibody
- CBP antibody
- eIF 4E antibody
see all
图片
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Western blot - Anti-eIF4E antibody (ab72116)All lanes : Anti-eIF4E antibody (ab72116) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK-1 cell lysate
Lane 5 : NIH3T3 cell lysate
Predicted band size: 25 kDaDetection: Chemiluminescence with an exposure time of 10 seconds.
Previous -
Immunoprecipitation - Anti-eIF4E antibody (ab72116)Detection of eIF4E by Western Blot of Immunprecipitate.
anti-eIF4E at 1µg/ml staining eIF4E in HeLa whole cell lysate immunoprecipitated using ab72116 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab72116 尚未被引用在任何文献中。