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Anti-eIF4B抗体(ab38359)

Submit a review Q&A (1)References (1)
Western blot - Anti-eIF4B antibody (ab38359)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody (ab38359)

Key features and details

  • Rabbit polyclonal to eIF4B
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-eIF4B抗体
    参阅全部 eIF4B 一抗

  • 描述

    兔多克隆抗体to eIF4B

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish

  • 免疫原

    Synthetic peptide corresponding to Human eIF4B aa 300-400 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab38358)

  • 阳性对照

    • Recombinant Human eIF4B protein (ab114511) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab38359于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ICC/IF
Use a concentration of 1 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).
ICC/IF
Use a concentration of 1 µg/ml.

靶标

图片

  • Western blot - Anti-eIF4B antibody (ab38359)
    Western blot - Anti-eIF4B antibody (ab38359)
    All lanes : Anti-eIF4B antibody (ab38359) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 69 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody (ab38359)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF4B antibody (ab38359)
    ICC/IF image of ab38359 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38359, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

文献 (1)

发表研究结果有使用 ab38359？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab38359 被引用在 1 文献中.

  • Wang F  et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490

客户评价及客户问答

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Question

I am interested in purchasing an anti-eIF4B antibody from you. It appears you have three such antibodies ab38359, ab66780, ab78916. I would like to use the antibody in western blot, immunofluorescence and IHC in human tissue and cell lines. Ideally I would like one antibody that could work in all three applications. ab78916 seems to be the most widely validated antibody in that images for these applications are available on your website. However, the IF image for ab38359 is much more convincing. Could you tell me the reason why you are marketing all three antibodies? Could you give me advice on which one to try?   Thanks very much for your help

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Answer

Thank you very much for contacting us regarding our eIF4B antibodies. We sell these three particular antibodies because they have been derived from different areas of human eIF4B. Ab78916 is derived from human eIF4B around the phosphorylation site of serine 422 (T-G-SP-E-S). Ab66780 corresponding to a region within C terminal amino acids 505-554 and ab38359 derived from within residues 300 - 400 of Human eIF4B. If an antibody has not been tested in an assay or one a species with >85% homology, I can offer a discount off a future purchase if you buy that now, test it and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. If you are not interested in creating an abreview, I would suggest using ab78916 as it has been tested for WB, IHC and ICC-IF. Our Abpromise ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I hope this information is helpful, but please do not hesitate to contact us with further questions.

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