重组Anti-eIF4A3抗体[EPR14302(B)] (ab180519)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14302(B)] to eIF4A3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-eIF4A3抗体[EPR14302(B)]
参阅全部 eIF4A3 一抗 -
描述
兔单克隆抗体[EPR14302(B)] to eIF4A3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: eIF4A3 Human Recombinant Protein and MCF7, HeLa, Raji, and HepG2 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR14302(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180519于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
靶标
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功能
ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
细胞定位
Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites. - Information by UniProt
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数据库链接
- Entrez Gene: 9775 Human
- Entrez Gene: 192170 Mouse
- Entrez Gene: 688288 Rat
- Omim: 608546 Human
- SwissProt: P38919 Human
- SwissProt: Q91VC3 Mouse
- SwissProt: Q3B8Q2 Rat
- Unigene: 389649 Human
see all -
别名
- ATP-dependent RNA helicase DDX48 antibody
- ATP-dependent RNA helicase eIF4A-3 antibody
- DDX48 antibody
see all
图片
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All lanes : Anti-eIF4A3 antibody [EPR14302(B)] (ab180519) at 1/1000 dilution
Lane 1 : eIF4A1 Human Recombinant Protein
Lane 2 : eIF4A2 Human Recombinant Protein
Lane 3 : eIF4A3 Human Recombinant Protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/dilution buffer and concentration: 5% NFDM/TBST. -
All lanes : Anti-eIF4A3 antibody [EPR14302(B)] (ab180519) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 47 kDa
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab180519 被引用在 1 文献中.
- Pacheco-Fiallos B et al. mRNA recognition and packaging by the human transcription-export complex. Nature 616:828-835 (2023). PubMed: 37020021