AB180506

重组Anti-eIF4A1抗体[EPR14505(B)] - N-terminal

Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal eIF4A1 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

DDX2A, EIF4A, EIF4A1, Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA helicase eIF4A-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (AB180506)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (AB180506)

Immunofluorescence analysis of MCF-7 cells labeling eIF4A1 (green) with ab180506 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor^® 488-conjugated goat anti rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (AB180506)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (AB180506)

Intracellular Flow Cytometry analysis of eIF4A1 expression in K562 cells using ab180506 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/75 dilution.

Supplier Data

Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (AB180506)

All lanes:

Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Supplier Data

Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (AB180506)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-eIF4A1 antibody [EPR14505(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14505(B)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic initiation factor 4A1 also known as eIF4A1 is an essential RNA helicase part of the DEAD-box family. It unwinds secondary structures in the 5' untranslated region of mRNA facilitating ribosome binding during translation initiation. This protein has a molecular mass of approximately 46 kDa. Expressed ubiquitously in eukaryotic cells eIF4A1 plays a central role in regulating protein synthesis by enhancing the accessibility of mRNA templates to the ribosome.

Biological function summary

The eIF4A1 protein functions as a component of the eIF4F complex which includes eIF4E and eIF4G. This complex modulates the initiation of mRNA translation a critical step in gene expression. By associating with other initiation factors eIF4A1 helps orchestrate the recruitment of the ribosome to capped mRNA. Its activity influences cell growth proliferation and response to environmental signals.

Pathways

EIF4A1 involves in the regulation of the mTOR signaling pathway which controls cell growth based on nutrient availability. It also participates in the integrated stress response pathway linking stress signals to translational control. eIF4A1 interacts with other proteins such as eIF4E and eIF4G through its function within these pathways highlighting its role in translation regulation.

EIF4A1 has been implicated in cancer and neurodegenerative diseases. Aberrant regulation of eIF4A1 expression and activity contributes to oncogenesis by promoting uncontrolled protein synthesis and cell proliferation. In neurodegenerative disorders altered eIF4A1 activity disrupts normal neuronal function and viability. Connections to proteins like eIF4E highlight its impact on disease progression emphasizing the importance of understanding eIF4A1's functional mechanisms in these contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome (PubMed : 20156963). In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth (PubMed : 20156963).

See full target information EIF4A1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:21180 PubMed40596687

2025

Pan-cancer analysis of oncogenic role of MAGOH and experiment validation in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zezhi Qiu,Yi Le,Yanyu Liao,Xiao Chen,Xing Xin,Fengting Nie,Xianpin Wei,Heng Wang,Ziling Fang

View all publications

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Abcam Product Promise

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重组Anti-eIF4A1抗体[EPR14505(B)] - N-terminal