Anti-eIF4A1抗体(ab31217)
Key features and details
- Rabbit polyclonal to eIF4A1
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-eIF4A1抗体
参阅全部 eIF4A1 一抗 -
描述
兔多克隆抗体to eIF4A1 -
宿主
Rabbit -
特异性
Replenishment batches of our polyclonal antibody, ab31217 are tested in WB. Previous batches were additionally validated in ICC/IF, IHC-P and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab185946.
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经测试应用
适用于: WB, IP, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human, African green monkey
预测可用于: Monkey
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) This antibody gave a positive signal in the following tissue lysates: Liver (Mouse) Pancreas (Mouse) Testis (Mouse)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (4) |
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
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| IP | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). |
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IP
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. -
序列相似性
Belongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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数据库链接
- Entrez Gene: 504958 Cow
- Entrez Gene: 1973 Human
- Entrez Gene: 13681 Mouse
- Entrez Gene: 287436 Rat
- Omim: 602641 Human
- SwissProt: Q3SZ54 Cow
- SwissProt: P60842 Human
- SwissProt: P60843 Mouse
see all -
别名
- ATP-dependent RNA helicase eIF4A-1 antibody
- DDX2 antibody
- DDX2A antibody
see all
图片
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Western blot - Anti-eIF4A1 antibody (ab31217)All lanes : Anti-eIF4A1 antibody (ab31217) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (ab31217)ICC/IF image of ab31217 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31217, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). -
Western blot - Anti-eIF4A1 antibody (ab31217)All lanes : Anti-eIF4A1 antibody (ab31217) at 1 µg/ml
Lane 1 : NIH/3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 4 :Mouse pancreas tissue lysate - total protein (ab29363)
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa -
Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)eIF4A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31217.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 47kDa: eIF4A1
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (59)
ab31217 被引用在 59 文献中.
- Drino A et al. Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs. Nucleic Acids Res 51:1326-1352 (2023). PubMed: 36718960
- Schmidt T et al. eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation. Nucleic Acids Res 51:1859-1879 (2023). PubMed: 36727461
- Steinmann SM et al. eIF4A1 Is a Prognostic Marker and Actionable Target in Human Hepatocellular Carcinoma. Int J Mol Sci 24:N/A (2023). PubMed: 36768380
- Ercan H et al. A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection. Cells 12:N/A (2023). PubMed: 36899884
- Volegova MP et al. The Helix-Loop-Helix motif of human EIF3A regulates translation of proliferative cellular mRNAs. PLoS One 18:e0292080 (2023). PubMed: 37768948