AB31217

Anti-eIF4A1 抗体

Name: eIF4A1抗体 (ab31217)| Abcam中文官网
Brand: Abcam Limited
SKU: AB31217
Price: 4359 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-eIF4A1 antibody

(6 Reviews)

(70 Publications)

Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody detecting eIF4A1 in Western Blot, IP, IHC-P, ICC/IF. Suitable for African green monkey, Cow, Human, Mouse, Rat.

- Over 50 publications
- Trusted since 2006

查看别名

DDX2A, EIF4A, EIF4A1, Eukaryotic initiation factor 4A-I, eIF-4A-I, eIF4A-I, ATP-dependent RNA helicase eIF4A-1

4 Images

Project2011****

Western blot - Anti-eIF4A1 antibody (AB31217)

All lanes:

Western blot - Anti-eIF4A1 antibody (ab31217) at 1 µg/mL

Lane 1:

Western blot - NIH/3T3 whole cell lysate (<a href='/products/cell-lysates/nih-3t3-whole-cell-lysate-ab7179'>ab7179</a>) at 10 µg

Lane 2:

MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 3:

Liver (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 4:

Western blot - Mouse pancreas tissue lysate - total protein (<a href='/products/tissue-lysates/mouse-pancreas-tissue-lysate-total-protein-ab29363'>ab29363</a>) at 10 µg

Lane 5:

Testis (Mouse) Tissue Lysate - normal tissue at 10 µg

Lane 6:

PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 46 kDa

Observed band size: 47 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (AB31217)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (AB31217)

ICC/IF image of ab31217 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab31217, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used to quench autofluorescence. 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

Unknown

Immunoprecipitation - Anti-eIF4A1 antibody (AB31217)

eIF4A1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to eIF4A1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab31217.
Secondary : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band : 47kDa : eIF4A1

All lanes:

Immunoprecipitation - Anti-eIF4A1 antibody (ab31217)

Predicted band size: 46 kDa

false

Project

Western blot - Anti-eIF4A1 antibody (AB31217)

All lanes:

Western blot - Anti-eIF4A1 antibody (ab31217) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 46 kDa

Observed band size: 47 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human, African green monkey, Cow

应用

IHC-P, IP, ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

Replenishment batches of our polyclonal antibody, ab31217 are tested in WB. Previous batches were additionally validated in ICC/IF, IHC-P and IP. These applications are still expected to work and are covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab185946.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-eIF4A1 antibody (ab31217) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Cow, Human, Mouse, Rat samples.

What is the molecular weight of eIF4A1?
Anti-eIF4A1 (ab31217) specifically detects a band for eIF4A1 (UniProt: P60842) at a molecular weight of 47kDa.

Trusted by the scientific community
Anti-eIF4A1 (ab31217) was first used in a scientific publication in 2006 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-eIF4A1 (ab31217) has over 5 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic initiation factor 4A1 also known as eIF4A1 is an essential RNA helicase part of the DEAD-box family. It unwinds secondary structures in the 5' untranslated region of mRNA facilitating ribosome binding during translation initiation. This protein has a molecular mass of approximately 46 kDa. Expressed ubiquitously in eukaryotic cells eIF4A1 plays a central role in regulating protein synthesis by enhancing the accessibility of mRNA templates to the ribosome.

Biological function summary

The eIF4A1 protein functions as a component of the eIF4F complex which includes eIF4E and eIF4G. This complex modulates the initiation of mRNA translation a critical step in gene expression. By associating with other initiation factors eIF4A1 helps orchestrate the recruitment of the ribosome to capped mRNA. Its activity influences cell growth proliferation and response to environmental signals.

Pathways

EIF4A1 involves in the regulation of the mTOR signaling pathway which controls cell growth based on nutrient availability. It also participates in the integrated stress response pathway linking stress signals to translational control. eIF4A1 interacts with other proteins such as eIF4E and eIF4G through its function within these pathways highlighting its role in translation regulation.

EIF4A1 has been implicated in cancer and neurodegenerative diseases. Aberrant regulation of eIF4A1 expression and activity contributes to oncogenesis by promoting uncontrolled protein synthesis and cell proliferation. In neurodegenerative disorders altered eIF4A1 activity disrupts normal neuronal function and viability. Connections to proteins like eIF4E highlight its impact on disease progression emphasizing the importance of understanding eIF4A1's functional mechanisms in these contexts.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome (PubMed : 20156963). In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth (PubMed : 20156963).

See full target information EIF4A1

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文献 (70)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40842239

2025

DHX8 regulates degradation of RNA by RNautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohei Sakai,Eigo Takeda,Chihana Kabuta,Viorica Raluca Contu,Yuuki Fujiwara,Nobuhiro Fujikake,Tadafumi Hashimoto,Yoshinori Ohsumi,Tomohiro Kabuta

Oncology reports 54: PubMed40682841

2025

High expression of eIF4A1 promotes angiogenesis through the NF‑κB/VEGFA pathway and predicts poor prognosis in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqun Zhu,Lizhou Jia,Xingwang Kuai,Qi Tang,Xinxia Chang,Xiao Zhang,Bing Chen,Hui Zhi,Haoran Hu,Xiaomei Huang,Zhenqing Feng,Wenbin Huang

Nucleic acids research 52:12039-12054 PubMed39225047

2024

Eukaryotic initiation factor 4B is a multi-functional RNA binding protein that regulates histone mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Quintas,Robert F Harvey,Emilie Horvilleur,Gavin D Garland,Tobias Schmidt,Lajos Kalmar,Veronica Dezi,Alberto Marini,Alexander M Fulton,Tuija A A Pöyry,Cameron H Cole,Martin Turner,Ritwick Sawarkar,Michael A Chapman,Martin Bushell,Anne E Willis

Clinical cancer research : an official journal of the American Association for Cancer Research 30:4464-4481 PubMed39078310

2024

Pharmacologic Inhibition of EIF4A Blocks NRF2 Synthesis to Prevent Osteosarcoma Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael M Lizardo,Christopher Hughes,Yue Z Huang,Taras Shyp,Alberto Delaidelli,Hai-Feng Zhang,Sol Snir Shaool,Annalena F Renner,Farez Burwag,Leanne C Sayles,Alex G Lee,Alejandro Sweet-Cordero,Poul H Sorensen

Molecular cell 84:2135-2151.e7 PubMed38848692

2024

eIF4F complex dynamics are important for the activation of the integrated stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Kyusik Q Kim,Ankanahalli N Nanjaraj Urs,Victor Lasehinde,Alison C Greenlaw,Benjamin H Hudson,Hani S Zaher

Journal of virology 98:e0006024 PubMed38557170

2024

PDCD4 restricts PRRSV replication in an eIF4A-dependent manner and is antagonized by the viral nonstructural protein 9.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiping Wei,Xiaoxiao Zhang,Xiaoying Wang,Lu Li,Yajie Fu,Yaosheng Chen,Xiaohong Liu,Chunhe Guo

PloS one 18:e0292080 PubMed37768948

2023

The Helix-Loop-Helix motif of human EIF3A regulates translation of proliferative cellular mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Marina P Volegova,Cynthia Hermosillo,Jamie H D Cate

Cells 12: PubMed36899884

2023

A Practical and Analytical Comparative Study of Gel-Based Top-Down and Gel-Free Bottom-Up Proteomics Including Unbiased Proteoform Detection.

Applications

Unspecified application

Species

Unspecified reactive species

Huriye Ercan,Ulrike Resch,Felicia Hsu,Goran Mitulovic,Andrea Bileck,Christopher Gerner,Jae-Won Yang,Margarethe Geiger,Ingrid Miller,Maria Zellner

Nucleic acids research 51:1859-1879 PubMed36727461

2023

eIF4A1-dependent mRNAs employ purine-rich 5'UTR sequences to activate localised eIF4A1-unwinding through eIF4A1-multimerisation to facilitate translation.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Schmidt,Adrianna Dabrowska,Joseph A Waldron,Kelly Hodge,Grigorios Koulouras,Mads Gabrielsen,June Munro,David C Tack,Gemma Harris,Ewan McGhee,David Scott,Leo M Carlin,Danny Huang,John Le Quesne,Sara Zanivan,Ania Wilczynska,Martin Bushell

Nucleic acids research 51:1326-1352 PubMed36718960

2023

Identification of RNA helicases with unwinding activity on angiogenin-processed tRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksej Drino,Lisa König,Charlotte Capitanchik,Nasim Sanadgol,Eva Janisiw,Tom Rappol,Elisa Vilardo,Matthias R Schaefer

View all publications

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Anti-eIF4A1 抗体

Anti-eIF4A1 antibody

Western blot - Anti-eIF4A1 antibody (AB31217)

Immunocytochemistry/ Immunofluorescence - Anti-eIF4A1 antibody (AB31217)

Immunoprecipitation - Anti-eIF4A1 antibody (AB31217)

Western blot - Anti-eIF4A1 antibody (AB31217)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性