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Anti-EIF3F抗体(ab64177)

Submit a review Q&A (2)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (ab64177)
  • Immunocytochemistry/ Immunofluorescence - Anti-EIF3F antibody (ab64177)

Key features and details

  • Rabbit polyclonal to EIF3F
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-EIF3F抗体
    参阅全部 EIF3F 一抗

  • 描述

    兔多克隆抗体to EIF3F

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Rat, Human
    预测可用于: Cynomolgus monkey

  • 免疫原

    Synthetic peptide:

    ARVIGTLLGTVD

    , corresponding to amino acids 114-125 of Human EIF3F
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab64177于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use a concentration of 2.5 µg/ml.
ICC/IF
Use at an assay dependent concentration.
说明
IHC-P
Use a concentration of 2.5 µg/ml.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
    Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.

  • 序列相似性

    Belongs to the eIF-3 subunit F family.
    Contains 1 MPN (JAB/Mov34) domain.

  • 结构域

    The MPN domain mediates deubiquitinating activity.

  • 翻译后修饰

    Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11.

  • 细胞定位

    Cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Deubiquitinating enzyme eIF3f antibody
      • eIF 3 epsilon antibody
      • eIF-3-epsilon antibody
      • eIF3 p47 antibody
      • eIF3 p47 subunit antibody
      • eIF3-epsilon antibody
      • eIF3-p47 antibody
      • eIF3f antibody
      • EIF3F_HUMAN antibody
      • EIF3S5 antibody
      • EIF3S5, formerly antibody
      • Eukaryotic translation initiation factor 3 subunit 5 antibody
      • Eukaryotic translation initiation factor 3 subunit 5, formerly antibody
      • Eukaryotic translation initiation factor 3 subunit F antibody
      • eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD) antibody
      • eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa antibody
      • eukaryotic translation initiation factor 3, subunit F antibody
      • translation initiation factor 3 47 kda subunit antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (ab64177)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (ab64177)
      ab64177 at 2.5µg/ml staining EIF3F in human brain cerebellum by Immunohistochemistry, Formalin-fixed, paraffin-embedded tissue.
    • Immunocytochemistry/ Immunofluorescence - Anti-EIF3F antibody (ab64177)
      Immunocytochemistry/ Immunofluorescence - Anti-EIF3F antibody (ab64177)
      ICC/IF image of ab64177 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64177, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    文献 (3)

    发表研究结果有使用 ab64177？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab64177 被引用在 3 文献中.

    • Theodoridis PR  et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33568529
    • Parajuli P  et al. Twist1 Activation in Muscle Progenitor Cells Causes Muscle Loss Akin to Cancer Cachexia. Dev Cell 45:712-725.e6 (2018). PubMed: 29920276
    • Lang CH  et al. BCATm deficiency ameliorates endotoxin-induced decrease in muscle protein synthesis and improves survival in septic mice. Am J Physiol Regul Integr Comp Physiol 299:R935-44 (2010). PubMed: 20554928

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Thank you for your email.
    Our customer would like to receive a credit note.
    Please arrange this.

    Best regards

    Read More
    Answer

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

    Credit ID: #####


    As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

    I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

    Read More

    Question

    Product code: 64177
    Lot number: GR30438-5

    Inquiry: No signal at predicted size. Please check this.


    1) Abcam product code :
    ab64177

    2) Abcam order reference number or product batch number :
    GR30438-5,

    2-1) storage temperature of antibody : -80℃

    3) Description of the problem :
    No signal

    4) Sample preparation:
    Type of sample and species(whole cell lysates, fraction, recombinant protein;and Human, mouse): Whole cell lysates , Human, SW480 cell line
    Lysis buffer : RIPA lysis beffer
    Protease inhibitors: 1x protesase inhibitor cocktail (sigma)
    Phosphatase inhibitors: 1x phosphatase inhibitor cocktail (sigma)
    Reducing agent: No
    Boiling for ≥5 min? yes (10min)
    Protein loaded ug/lane or cells/lane : 30ug/ lane
    Positive control : si-GFP sample and FLAG-eIF3f over expression sample
    Negative control : si-eIF3f sample

    5) Percentage of gel : 12% SDS-PAGE
    Type of membrane : PVDF membrane(Bio-rad)
    Protein transfer verified : Ponceau S
    Blocking agent and concentration : 5% Normal Horse Serum in 1x TBST
    Blocking time : 1hr
    Blocking temperature : Room Temperature

    6) Primary antibody (If more than one was used, describe in “additional notes”) :
    Concentration or dilution : 1:1000 and 1:500
    Diluent buffer : 5% Normal Horse Serum in 1x TBST
    Incubation time : 12hr
    Incubation temperature: 4℃

    7) Secondary antibody: peroxidase-conjugated Affinipure Donkey anti-Rabbit IgG(H+L) (711-035-152, Jackson ImmunoReserch)
    Species: Donkey
    Isotype: IgG
    Reacts against:
    Concentration or dilution : 1: 2000
    Diluent buffer : 1x TBST
    Incubation time : 1hr
    Incubation temperature: Room Temperature
    Fluorochrome or enzyme conjugate: HRP

    8) Washing after primary and secondary antibodies:
    Buffer : 1x TBST
    Number of washes : each 3 times for 10min

    9)Detection method : ECL solution method
    10) How many times have you run this staining? 3 times
    -Do you obtain the same results every time? 3 times
    -Have you run a No Primary; control? no
    -What steps have you altered to try and optimize the use of this antibody?
    I used low to high titer for this antibody. (1:1000 ˜1:500)



    Document attachment: Attaching images of your blot is strongly recommended and can greatly speed up our investigation of your problem.

    Our customer confirmed elF3f level through RT-PCR. But they didn't show any difference of elF3f level in endogenous, knock down modeled, over-expressed human sw480cell. also, they didn't show bands at predicted size.

    Read More
    Answer

    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will provide us with vital information for our monitoring of product quality

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been succesful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably recieved a bad vial.

    I apologise for the inconvenience and am pleased to offer you a free of charge replacement or credit note in compensation.

    In addition, I can suggest you may like to consider including a no primary control in future experiments, toassess if the secondary antibody is binding non specifically.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

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