Anti-EIF3F抗体(ab176853)
Key features and details
- Rabbit polyclonal to EIF3F
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-EIF3F抗体
参阅全部 EIF3F 一抗 -
描述
兔多克隆抗体to EIF3F -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide within Human EIF3F aa 307-357 (C terminal). The exact sequence is proprietary. NP_003745.1
Sequence:FLMS LVNQVPKIVP DDFETMLNSN INDLLMVTYL ANLTQSQIAL NEKLVNL
Database link: O00303 -
阳性对照
- WB: HeLa and NIH 3T3 whole cell lysates. IHC: Human ovarian carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176853 was affinity purified using an epitope specific to EIF3F immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176853于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
Use a concentration of 1 µg/ml. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 38 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling. -
序列相似性
Belongs to the eIF-3 subunit F family.
Contains 1 MPN (JAB/Mov34) domain. -
结构域
The MPN domain mediates deubiquitinating activity. -
翻译后修饰
Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 451007 Chimpanzee
- Entrez Gene: 513793 Cow
- Entrez Gene: 476840 Dog
- Entrez Gene: 100733279 Guinea pig
- Entrez Gene: 8665 Human
- Entrez Gene: 66085 Mouse
- Entrez Gene: 293427 Rat
- Entrez Gene: 706269 Rhesus monkey
see all -
别名
- Deubiquitinating enzyme eIF3f antibody
- eIF 3 epsilon antibody
- eIF-3-epsilon antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF3F antibody (ab176853)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue labelling eIF3F with ab176853 at 1 µg/mL.
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Western blot - Anti-EIF3F antibody (ab176853)All lanes : Anti-EIF3F antibody (ab176853) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 38 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-EIF3F antibody (ab176853)Detection of EIF3F in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176853 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab176853 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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Datasheet download
文献 (2)
ab176853 被引用在 2 文献中.
- V'kovski P et al. Determination of host proteins composing the microenvironment of coronavirus replicase complexes by proximity-labeling. Elife 8:N/A (2019). PubMed: 30632963
- Son YH et al. Dexamethasone downregulates caveolin-1 causing muscle atrophy via inhibited insulin signaling. J Endocrinol 225:27-37 (2015). WB ; Mouse . PubMed: 25688118
