AB248106

重组Anti-eIF3e抗体[EPR6877] - BSA and Azide free

Anti-eIF3e antibody [EPR6877] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal EIF3E antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

查看别名

EIF3S6, INT6, EIF3E, Eukaryotic translation initiation factor 3 subunit E, eIF3e, Eukaryotic translation initiation factor 3 subunit 6, Viral integration site protein INT-6 homolog, eIF-3 p48

2 Images

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Western blot - Anti-eIF3e antibody [EPR6877] - BSA and Azide free (AB248106)

This data was developed using ab125033, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-eIF3e antibody [EPR6877] (<a href='/products/primary-antibodies/eif3e-antibody-epr6877-ab125033'>ab125033</a>) at 1/1000 dilution

Lanes 1 and 6:

Jurkat cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

BxPC3 cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Lane 5:

Saos 2 cell lysate at 10 µg

Lane 7:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 48 kDa

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OI-RD Scanning - Anti-eIF3e antibody [EPR6877] - BSA and Azide free (AB248106)

OI-RD Scanning

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OI-RD Scanning - Anti-eIF3e antibody [EPR6877] - BSA and Azide free (AB248106)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Unconjugated

Anti-eIF3e antibody [EPR6877]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6877

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab248106 is the carrier-free version of ab125033.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EIF3e also known as eukaryotic translation initiation factor 3 subunit E is a critical component of the eIF3 complex. This protein has a mass of approximately 60 kDa and is expressed in a wide range of tissues. eIF3e plays a mechanical role in the translation initiation process facilitating the assembly of the pre-initiation complex by binding to the 40S ribosomal subunit and mRNA. It is essential for the correct positioning of mRNA in the ribosome enabling efficient start codon recognition.

Biological function summary

EIF3e participates in translation as part of the eIF3 complex which includes other subunits like eIF3a eIF3b and eIF3c. The complex facilitates ribosomal scanning and joining of the 60S ribosomal subunit promoting efficient protein synthesis. eIF3e specifically stabilizes the interaction of the initiation complex with mRNA ensuring accurate translation initiation. It is abundantly expressed in highly proliferative tissues reflecting its role in cell growth and division regulation.

Pathways

EIF3e plays a significant role in the mTOR signaling pathway and the Wnt signaling pathway. Both pathways regulate cell growth and proliferation which are important for development and tumorigenesis. eIF3e interacts with proteins like mTOR and beta-catenin that are integral to these pathways. It influences translation efficiency and has implications for protein synthesis regulation aligned with cellular growth cues.

EIF3e has connections with cancer and viral infections. Aberrant expression and mutations of eIF3e occur in various cancers affecting normal translation control and promoting tumorigenesis. eIF3e also relates to viral pathogenesis as some viruses hijack the host's translation machinery involving eIF3e for viral protein synthesis. These interactions can involve proteins like p53 in cancer where eIF3e's dysregulation contributes to cell cycle alterations and oncogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773). Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway (PubMed : 17468741). May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins (PubMed : 17310990, PubMed : 17324924).

See full target information EIF3E

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35269806

2022

Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Yu Chang,Hsuan-Miao Liu,Yann-Lii Leu,Chung-Hua Hsu,Tzung-Yan Lee

View all publications

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重组Anti-eIF3e抗体[EPR6877] - BSA and Azide free