重组Anti-eIF3e抗体[EPR6877] (ab125033)
![Western blot - Anti-eIF3e antibody [EPR6877] (ab125033)](https://www.abcam.cn/ps/products/125/ab125033/Images/eIF3e-Primary-antibodies-ab125033-1.jpg "Western blot - Anti-eIF3e antibody [EPR6877] (ab125033)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6877] to eIF3e
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-eIF3e抗体[EPR6877]
参阅全部 eIF3e 一抗 -
描述
兔单克隆抗体[EPR6877] to eIF3e -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
-
免疫原
Synthetic peptide within Human eIF3e aa 200-300. The exact sequence is proprietary.
-
阳性对照
- Jurkat, 293T, BxPC-3, A431, Saos 2, HepG2 and NIH 3T3 cell lysates
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 9.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6877 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa).
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa). |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
-
功能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins. -
组织特异性
Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus. -
序列相似性
Belongs to the eIF-3 subunit E family.
Contains 1 PCI domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus > PML body. - Information by UniProt
-
数据库链接
- Entrez Gene: 3646 Human
- Entrez Gene: 16341 Mouse
- Entrez Gene: 299872 Rat
- Omim: 602210 Human
- SwissProt: P60228 Human
- SwissProt: P60229 Mouse
- SwissProt: Q641X8 Rat
- Unigene: 405590 Human
see all -
别名
- eIF-3 p48 antibody
- eIF3e antibody
- EIF3E_HUMAN antibody
see all
图片
-
Western blot - Anti-eIF3e antibody [EPR6877] (ab125033)All lanes : Anti-eIF3e antibody [EPR6877] (ab125033) at 1/1000 dilution
Lanes 1 & 6 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : BxPC3 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : Saos 2 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-eIF3e antibody [EPR6877] (ab125033)](https://www.abcam.cn/ps/products/125/ab125033/Images/eIF3e-Primary-antibodies-ab125033-2.jpg)
OI-RD Scanning - Anti-eIF3e antibody [EPR6877] (ab125033) -
![Anti-eIF3e antibody [EPR6877] (ab125033)](https://www.abcam.cn/ps/products/125/ab125033/Images/ab125033-3-benefits-of-recombinant-antibodies.png)
Anti-eIF3e antibody [EPR6877] (ab125033)
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab125033 被引用在 1 文献中.
- Hoedt E et al. SILAC-based proteomic profiling of the human MDA-MB-231 metastatic breast cancer cell line in response to the two antitumoral lactoferrin isoforms: the secreted lactoferrin and the intracellular delta-lactoferrin. PLoS One 9:e104563 (2014). WB ; Human . PubMed: 25116916