重组Anti-eIF3B抗体[EPR5805] (ab124778)
![Western blot - Anti-eIF3B antibody [EPR5805] (ab124778)](https://www.abcam.cn/ps/products/124/ab124778/Images/ab124778-138647-anti-eif3b-antibody-epr5805-western-blot.jpg "Western blot - Anti-eIF3B antibody [EPR5805] (ab124778)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5805] to eIF3B
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-eIF3B抗体[EPR5805]
参阅全部 eIF3B 一抗 -
描述
兔单克隆抗体[EPR5805] to eIF3B -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human eIF3B aa 750-850. The exact sequence is proprietary.
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阳性对照
- WB: 293T, Jurkat, HeLa or Caco 2 cell lysates IHC-P: Human colon tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5805 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124778于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 92 kDa).
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 116 kDa (predicted molecular weight: 92 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IP.
靶标
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功能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
序列相似性
Belongs to the eIF-3 subunit B family.
Contains 1 RRM (RNA recognition motif) domain.
Contains 5 WD repeats. -
结构域
The RRM domain mediates interaction with EIF3J. -
翻译后修饰
Phosphorylated. Phosphorylation is enhanced upon serum stimulation. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8662 Human
- Omim: 603917 Human
- SwissProt: P55884 Human
- Unigene: 371001 Human
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别名
- eIF-3-eta antibody
- eIF3 eta antibody
- eIF3 p110 antibody
see all
图片
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Western blot - Anti-eIF3B antibody [EPR5805] (ab124778)All lanes : Anti-eIF3B antibody [EPR5805] (ab124778) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 92 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody [EPR5805] (ab124778)](https://www.abcam.cn/ps/products/124/ab124778/Images/ab124778-138646-anti-eif3b-antibody-epr5805-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3B antibody [EPR5805] (ab124778)ab124778, at 1/50, staining eIF3B in formalin fixed paraffin embedded Human colon tissue using immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-eIF3B antibody [EPR5805] (ab124778)](https://www.abcam.cn/ps/products/124/ab124778/Images/ab124778-3-benefits-of-recombinant-antibodies.png)
Anti-eIF3B antibody [EPR5805] (ab124778)
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab124778 被引用在 2 文献中.
- Ren H et al. Eukaryotic Translation Initiation Factor 3 Subunit B Is a Promoter in the Development and Progression of Pancreatic Cancer. Front Oncol 11:644156 (2021). PubMed: 33996561
- Zang Y et al. Eukaryotic Translation Initiation Factor 3b is both a Promising Prognostic Biomarker and a Potential Therapeutic Target for Patients with Clear Cell Renal Cell Carcinoma. J Cancer 8:3049-3061 (2017). PubMed: 28928896