重组Anti-eIF3B抗体[EPR5804] (ab133601)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5804] to eIF3B
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-eIF3B抗体[EPR5804]
参阅全部 eIF3B 一抗 -
描述
兔单克隆抗体[EPR5804] to eIF3B -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human eIF3B aa 150-250. The exact sequence is proprietary.
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阳性对照
- A431, 293T, Jurkat, HeLa and Caco 2 cell lysate; HeLa cells ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.60 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5804 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133601于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 116 kDa (predicted molecular weight: 92 kDa).
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ICC/IF |
1/500.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 116 kDa (predicted molecular weight: 92 kDa). |
ICC/IF
1/500. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. -
序列相似性
Belongs to the eIF-3 subunit B family.
Contains 1 RRM (RNA recognition motif) domain.
Contains 5 WD repeats. -
结构域
The RRM domain mediates interaction with EIF3J. -
翻译后修饰
Phosphorylated. Phosphorylation is enhanced upon serum stimulation. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8662 Human
- Entrez Gene: 27979 Mouse
- Entrez Gene: 288516 Rat
- Omim: 603917 Human
- SwissProt: P55884 Human
- SwissProt: Q8JZQ9 Mouse
- SwissProt: Q4G061 Rat
- Unigene: 371001 Human
see all -
别名
- eIF-3-eta antibody
- eIF3 eta antibody
- eIF3 p110 antibody
see all
图片
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All lanes : Anti-eIF3B antibody [EPR5804] (ab133601) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 92 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling eIF3B with purified ab133601 at 1/500 dilution (4.1 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain.
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Intracellular flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab133601 被引用在 8 文献中.
- Chen X et al. Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation. Cell Rep 39:110927 (2022). PubMed: 35675771
- Ye Y et al. Genome-wide identification and characterization of circular RNA m6A modification in pancreatic cancer. Genome Med 13:183 (2021). PubMed: 34798904
- Wang L et al. EIF3B is associated with poor outcomes in gastric cancer patients and promotes cancer progression via the PI3K/AKT/mTOR signaling pathway. Cancer Manag Res 11:7877-7891 (2019). PubMed: 31686906
- Sammaibashi S et al. Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins. Front Microbiol 9:1446 (2018). PubMed: 30008712
- Tang X et al. The Combination of piR-823 and Eukaryotic Initiation Factor 3 B (EIF3B) Activates Hepatic Stellate Cells via Upregulating TGF-ß1 in Liver Fibrogenesis. Med Sci Monit 24:9151-9165 (2018). PubMed: 30556540
- Osbun JW et al. Comparative Proteomic Profiling Using Two-Dimensional Gel Electrophoresis and Identification via LC-MS/MS Reveals Novel Protein Biomarkers to Identify Aggressive Subtypes of WHO Grade I Meningioma. J Neurol Surg B Skull Base 78:371-379 (2017). PubMed: 28875114
- Yue MM et al. Novel RNA-binding protein P311 binds eukaryotic translation initiation factor 3 subunit b (eIF3b) to promote translation of transforming growth factor ß1-3 (TGF-ß1-3). J Biol Chem 289:33971-83 (2014). ICC/IF ; Mouse . PubMed: 25336651
- Hu W et al. Cpeb4-mediated translational regulatory circuitry controls terminal erythroid differentiation. Dev Cell 30:660-72 (2014). PubMed: 25220394