AB133601

重组Anti-eIF3B抗体[EPR5804]

Anti-eIF3B antibody [EPR5804]

RabMAb
Recombinant
了解详情

(1 Review)

(9 Publications)

Rabbit Recombinant Monoclonal EIF3B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

查看别名

EIF3S9, EIF3B, Eukaryotic translation initiation factor 3 subunit B, eIF3b, Eukaryotic translation initiation factor 3 subunit 9, Prt1 homolog, eIF-3-eta, eIF3 p110, eIF3 p116, hPrt1

4 Images

Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] (AB133601)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-eIF3B antibody [EPR5804] (AB133601)

Intracellular flow cytometric analysis of permeabilized HeLa cells labelling eIF3B with ab133601 at 1/10 (red) with a Rabbit IgG control (green).

Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (AB133601)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-eIF3B antibody [EPR5804] (AB133601)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling eIF3B with purified ab133601 at 1/500 dilution (4.1 μg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain.

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Western blot - Anti-eIF3B antibody [EPR5804] (AB133601)

All lanes:

Western blot - Anti-eIF3B antibody [EPR5804] (ab133601) at 1/10000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 92 kDa

Observed band size: 116 kDa

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OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (AB133601)

OI-RD Scanning

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OI-RD Scanning - Anti-eIF3B antibody [EPR5804] (AB133601)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (2)

578 PE

PE Anti-eIF3B antibody [EPR5804]
Carrier free

Anti-eIF3B antibody [EPR5804] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR5804

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic initiation factor 3 subunit B (eIF3B) also known as EIF3S9 or p116 plays a central role in the initiation of translation. This protein has a mass of approximately 97 kDa and serves as an important component of the eIF3 complex important for assembling the 40S ribosomal subunit with mRNA and initiator tRNA. eIF3B is ubiquitously expressed in various tissues indicating its fundamental role in cellular protein synthesis.

Biological function summary

EIF3B functions as part of the large eIF3 complex which consists of 13 subunits. This complex is important for the recruitment of mRNA to the 40S ribosomal subunit playing an essential role in the start of translation. eIF3B directly contributes to stabilizing the interaction between mRNA and the ribosome promoting the accurate and efficient formation of the translation initiation complex. This function highlights the importance of eIF3B in regulating cellular protein levels.

Pathways

EIF3B participates in key processes of mRNA translation and is integral to the translation initiation pathway. It interacts with other translation initiation factors including eIF4F and eIF5 orchestrating the assembly and positioning of the ribosome for translation initiation. Moreover eIF3B is also involved in the mTOR signaling pathway linking nutrient sensing to protein synthesis regulation.

EIF3B has been associated with various types of cancer such as breast and prostate cancer where its increased expression correlates with tumor progression and poor prognosis. The dysfunction of eIF3B affects protein synthesis and cell proliferation contributing to oncogenesis. Furthermore eIF3B's role is connected to the dysregulation of mTOR pathways which is significant in cancer development. Its interaction with other proteins like eIF4E and eIF5 signifies its impact on cancer progression pathways.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed : 17581632, PubMed : 25849773, PubMed : 27462815, PubMed : 9388245). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2 : GTP : methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed : 17581632, PubMed : 9388245). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed : 25849773).. (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed : 18056426).

See full target information EIF3B

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文献 (9)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:4231-4248 PubMed38760988

2024

Research on the mechanism of exosomes from different sources influencing the progression of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxian Mao,Jixian Liu

Cell reports 39:110927 PubMed35675771

2022

Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaona Chen,Guang Xue,Jieyu Zhao,Yuwei Zhang,Suyang Zhang,Wen Wang,Yang Li,Jie Yuan,Liangqiang He,Chun Yin Chan,Yan Liu,Wei Chen,Yu Zhao,Ping Hu,Hao Sun,Chun Kit Kwok,Huating Wang

Genome medicine 13:183 PubMed34798904

2021

Genome-wide identification and characterization of circular RNA mA modification in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Ye,Weiyi Feng,Jialiang Zhang,Kaiyu Zhu,Xudong Huang,Ling Pan,Jiachun Su,Yanfen Zheng,Rui Li,Shuang Deng,Ruihong Bai,Lisha Zhuang,Lusheng Wei,Junge Deng,Mei Li,Rufu Chen,Dongxin Lin,Zhixiang Zuo,Jian Zheng

Cancer management and research 11:7877-7891 PubMed31686906

2019

EIF3B is associated with poor outcomes in gastric cancer patients and promotes cancer progression via the PI3K/AKT/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Xianzi Wen,Fengming Luan,Tao Fu,Chao Gao,Hong Du,Ting Guo,Jing Han,Longtao Huangfu,Xiaojing Cheng,Jiafu Ji

Medical science monitor : international medical jo 24:9151-9165 PubMed30556540

2018

The Combination of piR-823 and Eukaryotic Initiation Factor 3 B (EIF3B) Activates Hepatic Stellate Cells via Upregulating TGF-β1 in Liver Fibrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuechan Tang,Xiaoli Xie,Xin Wang,Yan Wang,Xiaoyu Jiang,Huiqing Jiang

Frontiers in microbiology 9:1446 PubMed30008712

2018

Strain-Specific Contribution of Eukaryotic Elongation Factor 1 Gamma to the Translation of Influenza A Virus Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Sammaibashi,Seiya Yamayoshi,Yoshihiro Kawaoka

Journal of neurological surgery. Part B, Skull bas 78:371-379 PubMed28875114

2017

Comparative Proteomic Profiling Using Two-Dimensional Gel Electrophoresis and Identification via LC-MS/MS Reveals Novel Protein Biomarkers to Identify Aggressive Subtypes of WHO Grade I Meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua W Osbun,Philip D Tatman,Sumanpreet Kaur,Carolina Parada,Tina Busald,Luis Gonzalez-Cuyar,Min Shi,Donald E Born,Jing Zhang,Manuel Ferreira

The Journal of biological chemistry 289:33971-83 PubMed25336651

2014

Novel RNA-binding protein P311 binds eukaryotic translation initiation factor 3 subunit b (eIF3b) to promote translation of transforming growth factor β1-3 (TGF-β1-3).

Applications

ICC/IF

Species

Mouse

Michael M Yue,Kaosheng Lv,Stephen C Meredith,Jennifer L Martindale,Myriam Gorospe,Lucia Schuger

Developmental cell 30:660-72 PubMed25220394

2014

Cpeb4-mediated translational regulatory circuitry controls terminal erythroid differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Hu,Bingbing Yuan,Harvey F Lodish

View all publications

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重组Anti-eIF3B抗体[EPR5804]