重组Anti-eIF3A抗体[EPR5819(2)] (ab128996)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5819(2)] to eIF3A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-eIF3A抗体[EPR5819(2)]
参阅全部 eIF3A 一抗 -
描述
兔单克隆抗体[EPR5819(2)] to eIF3A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human eIF3A aa 1100-1200. The exact sequence is proprietary.
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阳性对照
- BxPC3, 293T, Jurkat, C6, RAW264.7, PC12 and L6 lysates; Human brain tissue; BxPC3 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 8.20 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5819(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab128996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Essential for the initiation of translation on type-1 viral ribosomal entry sites (IRESs), like for HCV, PV, EV71 or BEV translation (PubMed:23766293, PubMed:24357634). In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2 (PubMed:18056426). -
序列相似性
Belongs to the eIF-3 subunit A family.
Contains 1 PCI domain. -
翻译后修饰
Phosphorylated. Phosphorylation is enhanced upon serum stimulation. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8661 Human
- Entrez Gene: 13669 Mouse
- Entrez Gene: 292148 Rat
- Omim: 602039 Human
- SwissProt: Q14152 Human
- SwissProt: P23116 Mouse
- SwissProt: Q1JU68 Rat
- Unigene: 523299 Human
see all -
别名
- centrosomin homolog antibody
- cytoplasmic protein p167 antibody
- eIF 3 theta antibody
see all
图片
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All lanes : Anti-eIF3A antibody [EPR5819(2)] (ab128996) at 1/1000 dilution
Lane 1 : BxPC-3 (Human pancreas adenocarcinoma cell line) whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 5 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 7 : L6 (Rat skeletal muscle cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 166 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF3A antibody [EPR5819(2)] (ab128996)
ab128996, at 1/50 dilution, staining eIF3A in paraffin-embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128996, at 1/250 dilution, staining eIF3A in BxPC3 cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab128996 被引用在 7 文献中.
- Shi HZ et al. N6-methyladenosine reader YTHDF3 regulates melanoma metastasis via its 'executor'LOXL3. Clin Transl Med 12:e1075 (2022). PubMed: 36324258
- Mei C et al. eIF3a Regulates Colorectal Cancer Metastasis via Translational Activation of RhoA and Cdc42. Front Cell Dev Biol 10:794329 (2022). PubMed: 35300416
- Wang J et al. WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA. Aging (Albany NY) 13:11135-11149 (2021). PubMed: 33819187
- Jiang SL et al. The Downregulation of eIF3a Contributes to Vemurafenib Resistance in Melanoma by Activating ERK via PPP2R1B. Front Pharmacol 12:720619 (2021). PubMed: 34512348
- Chen T et al. MicroRNA-875-5p inhibits tumor growth and metastasis of hepatocellular carcinoma by targeting eukaryotic translation initiation factor 3 subunit a. Oncol Rep 44:2067-2079 (2020). PubMed: 33000235
- Sonobe Y et al. Translation of dipeptide repeat proteins from the C9ORF72 expanded repeat is associated with cellular stress. Neurobiol Dis 116:155-165 (2018). PubMed: 29792928
- Luo CH et al. HE4 and eIF3a Expression Correlates with Surgical Outcome and Overall Survival in Ovarian Cancer Patients with Secondary Cytoreduction. J Cancer 9:2472-2479 (2018). PubMed: 30026845