Anti-EIF2S1 (phospho S49)抗体(ab131489)
Key features and details
- Rabbit polyclonal to EIF2S1 (phospho S49)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EIF2S1 (phospho S49)抗体
参阅全部 EIF2S1 一抗 -
描述
兔多克隆抗体to EIF2S1 (phospho S49) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human EIF2S1 aa 1-100 (phospho S49) conjugated to keyhole limpet haemocyanin.
Database link: NP_004085.1 -
阳性对照
- HeLa cell lysate Human breast carcinoma tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131489 was purified by affinity-chromatography using an-epitope specific phosphopeptide. Non-phosphopeptide specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131489于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/100 - 1/200. (methanol fixed)
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/100 - 1/200. (methanol fixed) |
靶标
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功能
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
序列相似性
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
翻译后修饰
Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
细胞定位
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
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数据库链接
- Entrez Gene: 1965 Human
- Omim: 603907 Human
- SwissProt: P05198 Human
- Unigene: 151777 Human
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别名
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
图片
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All lanes : Anti-EIF2S1 (phospho S49) antibody (ab131489) at 1/500 dilution
Lane 1 : Extracts of Hela cells untreated
Lane 2 : Extracts of Hela cells treated with starvation
Predicted band size: 36 kDa -
Immunofluorescence analysis of methanol-fixed HeLa cells labelling EIF2S1 using ab131489, at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EIF2S1 (phospho S49) antibody (ab131489)Immunohistochemistry analysis of Human breast cancer labelling EIF2S1 using ab131489, at 1/100 dilution (Left) or preincubated with blocking peptide (right).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131489 尚未被引用在任何文献中。