Anti-EIF2S1抗体[EIF2S1A2B8] (ab50733)
Key features and details
- Mouse monoclonal [EIF2S1A2B8] to EIF2S1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
-
产品名称
Anti-EIF2S1抗体[EIF2S1A2B8]
参阅全部 EIF2S1 一抗 -
描述
小鼠单克隆抗体[EIF2S1A2B8] to EIF2S1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant Human EIF2S1
-
阳性对照
- HEK293 and NIH3T3 whole cell lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading...
-
纯度
Protein G purified -
纯化说明
ab50733 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
克隆
单克隆 -
克隆编号
EIF2S1A2B8 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50733于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/50. Predicted molecular weight: 36 kDa.
|
|
ICC/IF |
1/1.
|
说明 |
---|
WB
1/50. Predicted molecular weight: 36 kDa. |
ICC/IF
1/1. |
靶标
-
功能
Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. -
序列相似性
Belongs to the eIF-2-alpha family.
Contains 1 S1 motif domain. -
翻译后修饰
Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. -
细胞定位
Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). - Information by UniProt
-
数据库链接
- Entrez Gene: 1965 Human
- Entrez Gene: 13665 Mouse
- Omim: 603907 Human
- SwissProt: P05198 Human
- SwissProt: Q6ZWX6 Mouse
- Unigene: 151777 Human
- Unigene: 196220 Mouse
-
别名
- EIF 2 alpha antibody
- EIF 2 antibody
- EIF 2A antibody
see all
图片
-
Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + HEK293 whole cell lysate at 50 µg
Secondary
Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
ab50733 at 1/1 dilution staining human EIF2S1 in HeLa Cells by Immunocytochemistry. A 1/200 dilution of Alexa Fluor 488 anti-mouse IgG is used as a secondary.
-
Anti-EIF2S1 antibody [EIF2S1A2B8] (ab50733) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-Mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
-
Datasheet download
文献 (1)
ab50733 被引用在 1 文献中.
- Yu Y et al. PKR-like endoplasmic reticulum kinase is necessary for lipogenic activation during HCMV infection. PLoS Pathog 9:e1003266 (2013). WB ; Human . PubMed: 23592989