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Epigenetics and Nuclear Signaling DNA / RNA Translation Regulation

Anti-EIF2S1抗体(ab26197)

  • Datasheet
  • SDS
Reviews (6)Q&A (7)References (44)

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Western blot - Anti-EIF2S1 antibody (ab26197)
  • Western blot - Anti-EIF2S1 antibody (ab26197)
  • Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody (ab26197)

Key features and details

  • Rabbit polyclonal to EIF2S1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human, Drosophila melanogaster
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-EIF2S1 antibody [EPR23098-50] (ab242148)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-EIF2S1抗体
    参阅全部 EIF2S1 一抗
  • 描述

    兔多克隆抗体to EIF2S1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Human, Drosophila melanogaster
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human EIF2S1 aa 50-150 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human eIF2 alpha.

    参阅Abcam的专有抗源政策
  • 阳性对照

    • Recombinant Human EIF2S1 protein (ab123468) can be used as a positive control in WB. HeLa Whole Cell Lysate Jurkat Whole Cell Lysate A431 Whole Cell Lysate NIH 3T3 Whole Cell Lysate MEF1 Whole Cell Lysate
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab26197于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 1 µg/ml.
WB (6)
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
说明
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use at an assay dependent concentration. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

靶标

  • 功能

    Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • 序列相似性

    Belongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • 翻译后修饰

    Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • 细胞定位

    Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Target information above from: UniProt accession P05198 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 32617 Drosophila melanogaster
    • Entrez Gene: 1965 Human
    • Entrez Gene: 13665 Mouse
    • Entrez Gene: 54318 Rat
    • Omim: 603907 Human
    • SwissProt: P41374 Drosophila melanogaster
    • SwissProt: P05198 Human
    • SwissProt: Q6ZWX6 Mouse
    • SwissProt: P68101 Rat
    • Unigene: 3157 Drosophila melanogaster
    • Unigene: 151777 Human
    • Unigene: 196220 Mouse
    • Unigene: 1488 Rat
    see all
  • 别名

    • EIF 2 alpha antibody
    • EIF 2 antibody
    • EIF 2A antibody
    • EIF 2alpha antibody
    • eIF-2-alpha antibody
    • eIF-2A antibody
    • EIF-2alpha antibody
    • EIF2 alpha antibody
    • EIF2 antibody
    • EIF2A antibody
    • EIF2S1 antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody
    • Eukaryotic translation initiation factor 2 subunit 1 alpha antibody
    • Eukaryotic translation initiation factor 2 subunit 1 antibody
    • Eukaryotic translation initiation factor 2 subunit alpha antibody
    • IF2A_HUMAN antibody
    see all

图片

  • Western blot - Anti-EIF2S1 antibody (ab26197)
    Western blot - Anti-EIF2S1 antibody (ab26197)
    All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A-431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    ab26197 detects a band of ~ 36 kDa in HeLa, Jurkat and A431 whole cell lysates. This corresponds to the predicted molecular weight for eIF2 alpha according to Swiss prot. A number of other bands are detected at ~ 48, 23 and 15 kDa. We do not know the identity of these bands and we predict they represent non-specific binding.

  • Western blot - Anti-EIF2S1 antibody (ab26197)
    Western blot - Anti-EIF2S1 antibody (ab26197)
    All lanes : Anti-EIF2S1 antibody (ab26197) at 1 µg/ml

    Lane 1 : NIH/3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody (ab26197)
    Immunocytochemistry/ Immunofluorescence - Anti-EIF2S1 antibody (ab26197)
    ICC/IF image of ab26197 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab26197, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (44)

发表研究结果有使用 ab26197?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab26197 被引用在 44 文献中.

  • Qu J  et al. Piezo1 suppression reduces demyelination after intracerebral hemorrhage. Neural Regen Res 18:1750-1756 (2023). PubMed: 36751801
  • Zhang B  et al. MSX1 Regulates Goat Endometrial Function by Altering the Plasma Membrane Transformation of Endometrial Epithelium Cells during Early Pregnancy. Int J Mol Sci 24:N/A (2023). PubMed: 36835532
  • Naït-Saïdi R  et al. The small compound Icerguastat reduces muscle defects in oculopharyngeal muscular dystrophy through the PERK pathway of the unfolded protein response. Open Biol 13:230008 (2023). PubMed: 37042114
  • Szaruga M  et al. Activation of the integrated stress response by inhibitors of its kinases. Nat Commun 14:5535 (2023). PubMed: 37684277
  • Gu W & Yang C Zinc oxide nanoparticles inhibit malignant progression and chemotherapy resistance of ovarian cancer cells by activating endoplasmic reticulum stress and promoting autophagy. Exp Ther Med 26:508 (2023). PubMed: 37840563
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 13 Abreviews or Q&A

Western blot abreview for Anti-EIF2S1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Toxoplasma gondii Cell lysate - whole cell (RH?ku80 (tachyzoites))
Gel Running Conditions
Reduced Denaturing (gel 12%)
Loading amount
80 µg
Specification
RH?ku80 (tachyzoites)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Matheus Bastos

Verified customer

提交于 Nov 12 2021

Western blot abreview for Anti-EIF2S1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (tedpole's head)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
8 µg
Specification
tedpole's head
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Kyung noh Yoon

Verified customer

提交于 May 09 2019

Western blot abreview for Anti-EIF2S1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pichia pastoris Cell lysate - whole cell (Pichia pastoris GS115 (his-) total lysate)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
4.4 µg
Specification
Pichia pastoris GS115 (his-) total lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 06 2018

Western blot abreview for Anti-EIF2S1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - whole (2-week-old seedlings)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
40 µg
Specification
2-week-old seedlings
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 03 2015

Question

Could you please find me an antibody that detects total eIF2alpha protein and no just the phosphorylated ones.

Read More

Abcam community

Verified customer

Asked on Mar 01 2013

Answer

ab26197 is known to detect total protein levels of EIF2S1.

Read More

Abcam Scientific Support

回复于 Mar 01 2013

Question

I tried the new vial of the anti-eiF2 (ab26197) that you sent to me to replace the old one. But unfortunately it is also not working. I am sending in attached file a figure that shows that. The membrane is the same but was cut for hybridization with eiF2 and GADD34 and then with beta-tubulin and as you can see the last two worked well for tumors' samples, although GADD34 didn't work for cell line but I think is a because of lower quantity. We tried with different sample concentration, from 25 ug to 200ug, with different concentration of BSA and milk for blocking and antibody dilution and also different time of incubation and we never got a single band with anti-eiF2. Since abcam doesn't have a new lot number, could you please give us a credit note?

Many thanks in advance. I am waiting for your reply.

Best regards,

Read More

Abcam community

Verified customer

Asked on Oct 03 2012

Answer

Thank you for your message and update.

I am sorry to hear the replacement vial has also not worked. I appreciate the time you have spent on these experiments and it is regrettable the results have not been successful.

As requested, I have asked our Finance department to issue a credit note for you.

Credit note ID: ####

The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice. Please do not hesitate to contact me if you have any further questions or concerns.

Read More

Abcam Scientific Support

回复于 Oct 03 2012

Question

Thanks again for your reply. I was looking to the abcam's webpage and there is another anti-eIF2 antibody (ab5369). Do you know if this is more sensitive? If it is the same, you can send me another aliquot of the ab26197 and I'll try it, if is not a problem for you. You can send it for the same address of the last order.

Many thanks for your help.

Sincerely,

Read More

Abcam community

Verified customer

Asked on Aug 28 2012

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1153650.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our 6 month Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

With regards to ab5369, I am sorry we do not have any data to confirm if this antibody would be more sensitive. I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, anyEIF2S1 antibody tested in the species and application you are using should be suitable for your applications. Also, although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

回复于 Aug 28 2012

Question

I really need this antibody. So, could you please send a new one from a different lot? Or you need to do a credit note and we order again the antibody? Do you have the same antibody more specific? If for mouse samples.

Many thanks again for your fast reply. I am waiting your reply.

Sincerely,

Read More

Abcam community

Verified customer

Asked on Aug 28 2012

Answer

Thank you for your reply.

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

Therefore, we would be pleased to provide a replacement from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and you prefer a credit note in this case, I will be pleased to arrange this for you.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Aug 28 2012

Question

Please find in attached file the image that I got. The standard molecular weight from bottom to top is 30kDa, 46kDa, 58 kDa, 80 kDa and 175 kDa.

I already tried with less amount of sample, and with that I didn't get any band. The transfer was good because afterwards I incubated with tubulin and I got a really nice band. The samples were fresh and the secondary antibody was working perfectly since I did other blots with different primary antibodies and they worked well. I just didn't reduced the 2nd antibody, but if I have a very faint band with the right molecular weight and a strong with the wrong molecular weight, I think if I reduced the 2nd antibody I couldn't see the right band, is it right?

Thanks again for your help.

Sincerely,

Read More

Abcam community

Verified customer

Asked on Aug 24 2012

Answer

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a refund or credit note in compensation.

I look forward to hearing from you with details of how you would like to proceed.

Read More

Abcam Scientific Support

回复于 Aug 24 2012

Question

Here is thequestionnaire completed. Hope this could help to figure out what happens with this antibody.

Many thanks in advance. I am waiting your reply.

Sincerely,


Order Details
Antibody code: ab26197

Problem
Choose: Multiple bands and weak signal

Lot number: GR3277-1

General Information
Antibody storage conditions (temperature/reconstitution etc):-20ºC


Description of the problem (high background, wrong band size, more bands, no band etc.): Multiple bands and the right band size is weaker then all others.


Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.): protein extract


Sample preparation (Buffer/Protease inhibitors/Heating sample etc.): protein extract was obtained from mice tumors by using the RIPA buffer with protease and phosphatase inhibitors.


Amount of protein loaded: ˜100ug


Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.): 12% SDSdenaturating gel


Transfer and blocking conditions (Buffer/time period, Blocking agent etc.): Transfer in 1x TGM 1h @100v. membrane was blocked 3hours in 5%BSA in TBS-T.


Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step): ab26197 antibody was diluted in 5%BSA in TBS-T to the concentration of 1.25ug/ul. Incubation was performed o/n @ 4ºC, afterwards membrane was washed 4x 5min @ RT with TBS-T



Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step): the secondary antibody was the goat anti-rabbit IRDye 680 from LI-COR Biosciences (cat# 926-32221) at 1/10000 dilutionin5%BSA in TBS-T. Incubation was performed 2h @ RT and membrane was washed 3x 5 min in TBS-T and the final wash step was done 15 min in TBS at RT


Detection method (ECL, ECLPlus etc.): Odissey


Positive and negative controls used (please specify): none



Optimization attempts (problem solving): I tried more time of incubation and I loaded more sample.
How many times have you tried the Western? 3 times


Have you run a "No Primary" control?No

Do you obtain the same results every time?No
e.g. are the background bands always in the same place?


What steps have you altered?

Additional Notes: When I loaded more sample and increased the incubation time I got the 3 bands showed in the picture because the first two attempts I didn't get anything.

Read More

Abcam community

Verified customer

Asked on Aug 23 2012

Answer

Thank you for taking the time to complete our questionnaire. The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. I would appreciate if you are able to provide an image which would help us to assess the results.

2. We recommend to load 20 - 30 ug of protein per lane of the gel to ensure it is not overloaded.

3. Could you confirm if the transfer to the membrane and quality of the sample assessed using a loading control?

4. I can recommend to try some fresh samples.

5. Could you confirm if thecurrent vial of secondary antibody working well with other primary antibodies? The concentration of secondary antibody may need to be reduced in order to help optimize the results.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

Abcam Scientific Support

回复于 Aug 23 2012

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