AB250411

重组Anti-EIF2B1抗体[EPR13894(B)] - BSA and Azide free

Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free

RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal EIF2B1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

EIF2BA, EIF2B1, Translation initiation factor eIF2B subunit alpha, eIF2B GDP-GTP exchange factor subunit alpha

2 Images

Supplier Data

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free (AB250411)

This data was developed using ab181186, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] (<a href='/products/primary-antibodies/eif2b1-antibody-epr13894b-ab181186'>ab181186</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] - BSA and Azide free (AB250411)

This data was developed using ab181186, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab181186 at 1/1000 labeling elF2B1 immunoprecipitated from MCF7 cell lysate using ab181186 at 1/50.

All lanes:

Western blot - Anti-EIF2B1 antibody [EPR13894(B)] (<a href='/products/primary-antibodies/eif2b1-antibody-epr13894b-ab181186'>ab181186</a>)

Predicted band size: 34 kDa

Observed band size: 34 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-EIF2B1 antibody [EPR13894(B)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13894(B)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab250411 is the carrier-free version of ab181186.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EIF2B1 also known as eukaryotic translation initiation factor 2B subunit alpha functions mechanically as a regulatory subunit in the eIF2B complex. This protein has a mass of approximately 38 kDa. EIF2B1 is expressed in various tissues throughout the body including the brain heart liver and muscle. It plays a critical role in protein synthesis by regulating the exchange of GDP for GTP on the eIF2 protein which is essential for initiating translation.

Biological function summary

EIF2B1 regulates protein synthesis by modulating the activity of the eIF2B complex which is a guanine nucleotide exchange factor. This complex comprises five subunits and EIF2B1 is integral for its function. Through regulation of eIF2 EIF2B1 indirectly controls the rate of translation initiation affecting cell growth and stress response mechanisms. In neurons EIF2B1’s activity influences neuronal development and maintenance.

Pathways

EIF2B1 functions prominently in both the unfolded protein response and integrated stress response pathways. These pathways respond to cellular stress by altering translation rates and are essential for maintaining protein homeostasis. EIF2B1 interacts primarily through these pathways with other proteins such as eIF2 and ATF4 which is a transcription factor activated during stress responses.

EIF2B1 plays a significant role in disorders like Vanishing White Matter (VWM) disease. Mutations in the EIF2B1 gene can lead to VWM a genetic condition affecting the central nervous system and presenting symptoms such as ataxia and neurological deterioration. Additionally disruption of EIF2B1 function relates to stress-related disorders due to its role in stress response pathways. Through these pathways EIF2B1 associates with proteins like eIF2 which can also affect susceptibility to stress disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed : 25858979, PubMed : 27023709, PubMed : 31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed : 25858979, PubMed : 31048492).

See full target information EIF2B1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao

View all publications

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重组Anti-EIF2B1抗体[EPR13894(B)] - BSA and Azide free