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重组RabMAb

重组Anti-eIF1A抗体[EPR12467] (ab172623)

Submit a review Submit a question References (3)
Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)
  • Anti-eIF1A antibody [EPR12467] (ab172623)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12467] to eIF1A
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-eIF1A抗体[EPR12467]
    参阅全部 eIF1A 一抗

  • 描述

    兔单克隆抗体[EPR12467] to eIF1A

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IP, IHC-P, WBmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chimpanzee, Orangutan

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa, 293T, Jurkat, and A375 cell lysates; Colonic carcinoma tissue; Pancreas tissue; A375 cells; Immunoprecipitation pellet from HeLa whole cell lysate (ab150035)

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR12467

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab172623于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
说明
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 相关性

    eIF1A is an essential eukaryotic translation initiation factor. The protein is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA (referenced from entrez gene).

  • 细胞定位

    Cytoplasmic and Nuclear

  • 数据库链接

    see all

  • 别名

    • eIF-1A antibody
    • eIF-1A X isoform antibody
    • eIF-4C antibody
    • EIF1A antibody
    • EIF1AP1 antibody
    • EIF1AX antibody
    • EIF4C antibody
    • eukaryotic translation initiation factor 1A X-linked antibody
    • eukaryotic translation initiation factor 1A, X chromosome antibody
    • Eukaryotic translation initiation factor 1A, X-chromosomal antibody
    • eukaryotic translation initiation factor 4C antibody
    • Putative eukaryotic translation initiation factor 1A antibody
    see all

图片

  • Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
    Western blot - Anti-eIF1A antibody [EPR12467] (ab172623)
    All lanes : Anti-eIF1A antibody [EPR12467] (ab172623) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A375 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 16 kDa

  • Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunoprecipitation - Anti-eIF1A antibody [EPR12467] (ab172623)

    Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling eIF1A with ab172923 at 1/10 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling eIF1A with ab172623 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF1A antibody [EPR12467] (ab172623)

    Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue labeling eIF1A with ab172623 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)
    Immunocytochemistry/ Immunofluorescence - Anti-eIF1A antibody [EPR12467] (ab172623)

    Immunofluorescent analysis of A375 cells labeling eIF1A with ab172623 at 1/50 dilution (green) and counterstained with DAPI (blue)

  • Anti-eIF1A antibody [EPR12467] (ab172623)
    Anti-eIF1A antibody [EPR12467] (ab172623)

文献 (3)

发表研究结果有使用 ab172623？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab172623 被引用在 3 文献中.

  • Del Puerto-Nevado L  et al. Diabetes-mediated promotion of colon mucosa carcinogenesis is associated with mitochondrial dysfunction. Mol Oncol 13:1887-1897 (2019). PubMed: 31199051
  • González-Almela E  et al. The Initiation Factors eIF2, eIF2A, eIF2D, eIF4A, and eIF4G Are Not Involved in Translation Driven by Hepatitis C Virus IRES in Human Cells. Front Microbiol 9:207 (2018). PubMed: 29487587
  • Yi T  et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB, IP . PubMed: 26018492

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