重组Anti-eIF-6抗体[EPR6513] (ab133321)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6513] to eIF-6
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-eIF-6抗体[EPR6513]
参阅全部 eIF-6 一抗 -
描述
兔单克隆抗体[EPR6513] to eIF-6 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human eIF-6 aa 200-300 (C terminal). The exact sequence is proprietary.
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阳性对照
- HeLa, 293T, HepG2, and K562 cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 5.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6513 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133321于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
靶标
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功能
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. -
组织特异性
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). -
序列相似性
Belongs to the eIF-6 family. -
翻译后修饰
Phosphorylation at Ser-174 and Ser-175 promotes nuclear export. -
细胞定位
Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 3692 Human
- Entrez Gene: 16418 Mouse
- Entrez Gene: 305506 Rat
- Omim: 602912 Human
- SwissProt: P56537 Human
- SwissProt: O55135 Mouse
- SwissProt: Q3KRD8 Rat
- Unigene: 654848 Human
see all -
别名
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
see all
图片
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All lanes : Anti-eIF-6 antibody [EPR6513] (ab133321) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 27 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab133321 尚未被引用在任何文献中。