Anti-eIF-6抗体(ab109593)
Key features and details
- Rabbit polyclonal to eIF-6
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-eIF-6抗体
参阅全部 eIF-6 一抗 -
描述
兔多克隆抗体to eIF-6 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Catfish, Rainbow trout, Orangutan
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免疫原
Synthetic peptide corresponding to Human eIF-6 aa 150-200.
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阳性对照
- HeLa or NIH3T3 whole cell lysate; IHC: mouse renal cell carcinoma (renca positive), human ovarian carcinoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109593于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. -
组织特异性
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). -
序列相似性
Belongs to the eIF-6 family. -
翻译后修饰
Phosphorylation at Ser-174 and Ser-175 promotes nuclear export. -
细胞定位
Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 286811 Cow
- Entrez Gene: 3692 Human
- Entrez Gene: 16418 Mouse
- Entrez Gene: 305506 Rat
- Entrez Gene: 100302338 Sheep
- Omim: 602912 Human
- SwissProt: Q9TU47 Cow
- SwissProt: P56537 Human
see all -
别名
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF-6 antibody (ab109593)
Immunohistochemistry analysis of formalin-fixed paraformaldehyde-fixed mouse renca positive renal cell carcinoma tissue staining eIF-6 with ab109593 with a 1/1000 dilution. Counterstained with hematoxylin
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eIF-6 antibody (ab109593)Immunohistochemistry analysis of formalin-fixed paraformaldehyde-fixed human ovarian carcinoma tissue staining eIF-6 with ab109593 with a 1/1000 dilution. Counterstained with hematoxylin
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Western blot - Anti-eIF-6 antibody (ab109593)All lanes : Anti-eIF-6 antibody (ab109593) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-eIF-6 antibody (ab109593)Detection of eIF-6 by Western Blot of Immunprecipitate.
Lane 1: ab109593 at 1µg/ml staining eIF-6 in HeLa whole cell lysate immunoprecipitated using ab109593 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab109593 尚未被引用在任何文献中。