重组Anti-EID1抗体[EPR13636] (ab180133)
![Western blot - Anti-EID1 antibody [EPR13636] (ab180133)](https://www.abcam.cn/ps/products/180/ab180133/Images/ab180133-210056-anti-eid1-antibody-epr13636-western-blot.jpg "Western blot - Anti-EID1 antibody [EPR13636] (ab180133)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13636] to EID1
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EID1抗体[EPR13636]
参阅全部 EID1 一抗 -
描述
兔单克隆抗体[EPR13636] to EID1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human cervix carcinoma tissue; HeLa and HepG2 whole cell lysate (ab7900).
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13636 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180133于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000. Predicted molecular weight: 21 kDa.
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| 说明 |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000. Predicted molecular weight: 21 kDa. |
靶标
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功能
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. -
组织特异性
Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines. -
发展阶段
Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level). -
翻译后修饰
Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. -
细胞定位
Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23741 Human
- Entrez Gene: 58521 Mouse
- Entrez Gene: 499882 Rat
- Omim: 605894 Human
- SwissProt: Q9Y6B2 Human
- SwissProt: Q9DCR4 Mouse
- Unigene: 255973 Human
- Unigene: 425101 Mouse
see all -
别名
- 21 kDa pRb associated protein antibody
- 21 kDa pRb-associated protein antibody
- C15orf3 antibody
see all
图片
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Western blot - Anti-EID1 antibody [EPR13636] (ab180133)All lanes : Anti-EID1 antibody [EPR13636] (ab180133) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 21 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EID1 antibody [EPR13636] (ab180133)](https://www.abcam.cn/ps/products/180/ab180133/Images/ab180133-210057-anti-eid1-antibody-epr13636-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EID1 antibody [EPR13636] (ab180133)Immunohistochemical analysis of paraffin-embedded human cervix carcinoma tissue labeling EID1 using ab180133 at 1/100 dilution. A Goat anti rabbit IgG (HRP) prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-EID1 antibody [EPR13636] (ab180133)](https://www.abcam.cn/ps/products/180/ab180133/Images/ab180133-3-benefits-of-recombinant-antibodies.png)
Anti-EID1 antibody [EPR13636] (ab180133)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab180133 尚未被引用在任何文献中。