Anti-EID1抗体[26] (ab78323)
![Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (ab78323)](https://www.abcam.cn/ps/products/78/ab78323/Images/ab78323-220780-78323-IF.jpg "Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (ab78323)")
Key features and details
- Mouse monoclonal [26] to EID1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-EID1抗体[26]
参阅全部 EID1 一抗 -
描述
小鼠单克隆抗体[26] to EID1 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Cow, Cynomolgus monkey, Orangutan
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免疫原
Synthetic peptide:
YEKTPFDQLAFIEELFSLMVVNRLTEELGC
, corresponding to amino acids 159 - 187 of Human EID1 -
阳性对照
- extracts from MCF7 cells transfected with EID1
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
ab78323 was purified by propriety chromatography under mild conditions as IgG fraction from serum free growth medium of mouse hybridoma clone and sterilized by filtration. -
克隆
单克隆 -
克隆编号
26 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78323于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa.
|
| 说明 |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 21 kDa. |
靶标
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功能
Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms. -
组织特异性
Widely expressed. Most abundantly expressed in heart, skeletal muscle, pancreas, brain and testis. Expressed at much lower levels in placenta and peripheral blood leukocyte. Barely detectable in lung. Also weakly expressed in lung carcinoma A549 and various leukemia cell lines. -
发展阶段
Expression decreased with development in ventricular tissue while remaining highly expressed in adult atrial tissue. In primary cultures of human skeletal myocytes, expression decreased during myogenic differentiation (at protein level). -
翻译后修饰
Ubiquitinated in U-2OS osteosarcoma cells and is rapidly degraded by proteasome as cells exit the cell cycle exit. -
细胞定位
Nucleus. Cytoplasm. May shuttle between nucleus and cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 23741 Human
- Omim: 605894 Human
- SwissProt: Q9Y6B2 Human
- Unigene: 255973 Human
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别名
- 21 kDa pRb associated protein antibody
- 21 kDa pRb-associated protein antibody
- C15orf3 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-EID1 antibody [26] (ab78323)
ab78323 staining EID1 in HeLA cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100 0.25% in PBS and blocked with 1.5% BSA for 30 minutes. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) overnight at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/1000 in PBS + 1% BSA) was used as the secondary antibody incubated for 1 hour. DAPI was used to stain the nuclear DNA.
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![Western blot - Anti-EID1 antibody [26] (ab78323)](https://www.abcam.cn/ps/products/78/ab78323/Images/EID1-Primary-antibodies-ab78323-7.JPG)
Western blot - Anti-EID1 antibody [26] (ab78323)All lanes : Anti-EID1 antibody [26] (ab78323) at 1 µg/ml
Lane 1 : extracts from MCF7 cells transfected with control
vector pCMV1
Lane 2 : extracts from MCF7 cells transfected with the EID1 expression
vector pcDNA3/EID1
Secondary
All lanes : HRP-conjugated mouse IgG
Predicted band size: 21 kDa
Observed band size: 21 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab78323 尚未被引用在任何文献中。