Anti-EHHADH抗体[7F6AF11] (ab110299)
![Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)](https://www.abcam.cn/ps/products/110/ab110299/Images/ab110299-123544-anti-ehhadh-antibody-7f6af11-immunoprecipitation.gif "Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)")
Key features and details
- Mouse monoclonal [7F6AF11] to EHHADH
- Suitable for: IP, ICC
- Reacts with: Human
- Isotype: IgG3
概述
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产品名称
Anti-EHHADH抗体[7F6AF11]
参阅全部 EHHADH 一抗 -
描述
小鼠单克隆抗体[7F6AF11] to EHHADH -
宿主
Mouse -
经测试应用
适用于: IP, ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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阳性对照
- ICC: HepG2 cells. IP: Enriched subcellular organelles from human liver.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Near homogeneity as judged by SDS-PAGE (>95% purity). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
克隆
单克隆 -
克隆编号
7F6AF11 -
同种型
IgG3 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110299于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at an assay dependent dilution.
|
|
| ICC |
Use a concentration of 1 µg/ml.
|
| 说明 |
|---|
|
IP
Use at an assay dependent dilution. |
|
ICC
Use a concentration of 1 µg/ml. |
靶标
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组织特异性
Liver and kidney. Lower amounts seen in the brain. -
通路
Lipid metabolism; fatty acid beta-oxidation. -
序列相似性
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. -
翻译后修饰
Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TSA) or with nicotinamide (NAM) with highest increase on Lys-346. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids. -
细胞定位
Peroxisome. - Information by UniProt
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数据库链接
- Entrez Gene: 1962 Human
- Omim: 607037 Human
- SwissProt: Q08426 Human
- Unigene: 429879 Human
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别名
- 3 hydroxyacyl CoA dehydrogenase antibody
- 3,2 trans enoyl CoA isomerase antibody
- 3-hydroxyacyl-CoA dehydrogenase antibody
see all
图片
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Immunoprecipitation - Anti-EHHADH antibody [7F6AF11] (ab110299)
Immonprecipitation analysis of enriched subcellular organelles from human liver, which contains mitochondria and peroxisome. EHHADH immunocaptured using ab110299.
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![Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (ab110299)](https://www.abcam.cn/ps/products/110/ab110299/Images/ab110299-123543-anti-ehhadh-antibody-7f6af11-immunocytochemistry.gif)
Immunocytochemistry - Anti-EHHADH antibody [7F6AF11] (ab110299)Immunocytochemistry image of EHHADH stained Human HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated ab110299 at 1µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in peroxisome.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab110299 尚未被引用在任何文献中。