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重组RabMAb

重组Anti-EHD1抗体[EPR4954] (ab109311)

Reviews (1)Q&A (1)References (24)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Immunoprecipitation - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
  • Anti-EHD1 antibody [EPR4954] (ab109311)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4954] to EHD1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-EHD1抗体[EPR4954]
    参阅全部 EHD1 一抗

  • 描述

    兔单克隆抗体[EPR4954] to EHD1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HepG2, Human testis, SW480, SK-OV-3, Human brain, HeLa, Mouse brain, and Rat brain lysates. IHC-P: human thyroid carcinoma, kidney and tonsil, mouse lung, and rat lung tissues. IP: HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab109311于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
IP
1/10 - 1/100.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98°C, allow to cool for 10-20 minutes)

1:120 in human tissues / 1:1600 in mouse and rat tissues

See IHC antigen retrieval protocols.’
说明
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 61 kDa).
IP
1/10 - 1/100.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

(heat to 98°C, allow to cool for 10-20 minutes)

1:120 in human tissues / 1:1600 in mouse and rat tissues

See IHC antigen retrieval protocols.’

靶标

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling EHD1 with purified ab109311 at 1/120 dilution (2.85 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunoprecipitation - Anti-EHD1 antibody [EPR4954] (ab109311)

    ab109311 (purified ) at 1/30 dilution (2ug) immunoprecipitating EHD1 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab109311 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109311 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Anti-EHD1 antibody [EPR4954] (ab109311) at 1/10000 dilution (Purified) + Human brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    Western blot - Anti-EHD1 antibody [EPR4954] (ab109311)
    All lanes : Anti-EHD1 antibody [EPR4954] (ab109311) at 1/1000 dilution ((unpurified))

    Lane 1 : HepG2 cell lysates
    Lane 2 : Human testis lysates
    Lane 3 : SW480 cell lysates
    Lane 4 : SK-OV-3 cell lysates
    Lane 5 : HeLa cell lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labeling EHD1 with purified ab109311 at 1/1600 dilution (0.21 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemical analysis of EHD1 in paraffin embedded Human kidney tissue, using unpurified ab109311 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHD1 antibody [EPR4954] (ab109311)

    Immunohistochemical analysis of EHD1 in paraffin embedded Human tonsil tissue, using unpurified ab109311 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-EHD1 antibody [EPR4954] (ab109311)
    Anti-EHD1 antibody [EPR4954] (ab109311)

文献 (24)

发表研究结果有使用 ab109311？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109311 被引用在 24 文献中.

  • Aslanyan MG  et al. A targeted multi-proteomics approach generates a blueprint of the ciliary ubiquitinome. Front Cell Dev Biol 11:1113656 (2023). PubMed: 36776558
  • Gouveia Roque C  et al. CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology. Sci Adv 9:eadd2671 (2023). PubMed: 36867706
  • Xie S  et al. EHD1 promotes CP110 ubiquitination by centriolar satellite delivery of HERC2 to the mother centriole. EMBO Rep 24:e56317 (2023). PubMed: 37074924
  • Chakraborty S  et al. EHD1-dependent traffic of IGF-1 receptor to the cell surface is essential for Ewing sarcoma tumorigenesis and metastasis. Commun Biol 6:758 (2023). PubMed: 37474760
  • Meindl K  et al. A missense mutation in Ehd1 associated with defective spermatogenesis and male infertility. Front Cell Dev Biol 11:1240558 (2023). PubMed: 37900275
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-EHD1 antibody [EPR4954]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Hyunsoo Kim

Verified customer

提交于 Mar 11 2021

Question

I would like to use this Ab to detect EHD1 on a western blot. Could you tell me the peptide it was rasised against as I would like to ensure that it is in a region not present in EHD2, 3, and 4. Thank you.

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Answer

Thank you for contacting Abcam regarding our EHD1 antibody, ab109311. I have confirmed with the laboratory that this antibody is not predicted to cross react with other EHD proteins EHD2, 3, and 4. Sequence analysis shows that the immunogen shares no significant sequence identity with them. It shares 69% identity with EHD3. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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