Anti-EHBP1L1抗体(ab122557)
Key features and details
- Rabbit polyclonal to EHBP1L1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-EHBP1L1抗体 -
描述
兔多克隆抗体to EHBP1L1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: IEVGLLGVLG IETGAAEGAI LGTQEIASRD SGVPGLEADT TGIQVKEVGG SEVPEIATGT AETEILGTQE IASRSSGVPG LESEVAGAQE TEVGGSGISG, corresponding to amino acids of Human EHBP1L1.
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阳性对照
- ICC: A-431 cells. IHC-P: Human skeletal muscle, prostate and lymph node tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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序列相似性
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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数据库链接
- Entrez Gene: 254102 Human
- SwissProt: Q8N3D4 Human
- Unigene: 502867 Human
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别名
- DKFZp762C186 antibody
- EH domain-binding protein 1-like protein 1 antibody
- EH1L1_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-EHBP1L1 antibody (ab122557)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122557 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (ab122557)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skeletal muscle tissue labeling EHBP1L1 with ab122557 at 1/5000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (ab122557)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling EHBP1L1 with ab122557 at 1/5000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (ab122557)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymph node tissue labeling EHBP1L1 with ab122557 at 1/5000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHBP1L1 antibody (ab122557)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebral cortex tissue labeling EHBP1L1 with ab122557 at 1/5000. No positivity observed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab122557 被引用在 1 文献中.
- Pan Y et al. EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1. Adv Sci (Weinh) 10:e2206792 (2023). PubMed: 36775874