Anti-Egr1抗体(ab6054)
Key features and details
- Rabbit polyclonal to Egr1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Egr1抗体
参阅全部 Egr1 一抗 -
描述
兔多克隆抗体to Egr1该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。 Read the terms of use »
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宿主
Rabbit -
特异性
A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.
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种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide:
GEQPYEHLTAESFPDI-C
conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | |
| IHC-P |
| 说明 |
|---|
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. -
序列相似性
Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- Omim: 128990 Human
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
- SwissProt: P08154 Rat
- Unigene: 326035 Human
see all -
别名
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
see all
图片
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Western blot - Anti-Egr1 antibody (ab6054)Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Secondary
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (ab6054)ab6054 at 10 µg/ml staining human ovarian cancinoma.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab6054 被引用在 3 文献中.
- Inoue N et al. The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli. Elife 10:N/A (2021). PubMed: 33780330
- Zhao J et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). PubMed: 29393474
- Nadra K et al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB ; Mouse . PubMed: 18559480