Key features and details
- Rabbit polyclonal to Egr1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
参阅全部 Egr1 一抗
种属反应性与反应: Mouse, Human
GEQPYEHLTAESFPDI-Cconjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯度Immunogen affinity purified
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
序列相似性Belongs to the EGR C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
- Information by UniProt
- Entrez Gene: 1958 Human
- Entrez Gene: 13653 Mouse
- Entrez Gene: 24330 Rat
- Omim: 128990 Human
- SwissProt: P18146 Human
- SwissProt: P08046 Mouse
- SwissProt: P08154 Rat
- Unigene: 326035 Human
- AT225 antibody
- Early growth response 1 antibody
- Early growth response protein 1 antibody
Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
ab6054 at 10 µg/ml staining human ovarian cancinoma.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab6054 被引用在 3 文献中.
- Inoue N et al. The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli. Elife 10:N/A (2021). PubMed: 33780330
- Zhao J et al. REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells. Oncol Rep 39:1583-1590 (2018). PubMed: 29393474
- Nadra K et al. Phosphatidic acid mediates demyelination in Lpin1 mutant mice. Genes Dev 22:1647-61 (2008). WB ; Mouse . PubMed: 18559480