AB6054

Anti-Egr1抗体

Name: Egr1抗体 (ab6054)| Abcam中文官网
Brand: Abcam Limited
SKU: AB6054
Price: 4823 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Egr1 antibody

(1 Review)

(4 Publications)

Rabbit Polyclonal EGR1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human EGR1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

查看别名

KROX24, ZNF225, EGR1, Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, NGFI-A

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (AB6054)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (AB6054)

ab6054 at 10 μg/ml staining human ovarian cancinoma.

Unknown

Western blot - Anti-Egr1 antibody (AB6054)

All lanes:

Western blot - Anti-Egr1 antibody (ab6054) at 1/1500 dilution

All lanes:

Mouse embryonic fibroblast whole cell lysate at 35 µg

Secondary

All lanes:

Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 57 kDa

Observed band size: 58 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human EGR1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P18146

特异性

A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Egr1 also known as early growth response protein 1 is a zinc-finger transcription factor with a molecular weight of approximately 60 kDa. The Egr1 protein plays a significant role in gene expression regulation by binding to specific DNA sequences. This target is highly expressed in a variety of tissues including the brain heart and kidneys. In these tissues Egr1 regulates several cellular processes by activating or repressing target genes. Also Egr1 has monoclonal antibodies available for research purposes providing tools for detailed study in specific cellular contexts.

Biological function summary

Egr1 acts as an immediate-early response gene. It gets rapidly upregulated in response to growth factors stress signals and other extracellular stimuli. As part of the cellular signaling networks Egr1 interacts with other transcription factors to control the expression of genes involved in cell differentiation proliferation and apoptosis. It does not typically form part of a protein complex but may work in concert with other signaling molecules to influence diverse cellular pathways and maintain cellular homeostasis.

Pathways

Egr1 is involved in the MAPK/ERK and PI3K/AKT signaling pathways both key to regulating cellular growth and survival. It functions as a bridging factor that links extracellular signals to transcriptional changes within the nucleus. In the MAPK/ERK pathway Egr1 works alongside proteins like Ras and MEK to promote early gene transcription in response to mitogenic signals. In the PI3K/AKT pathway Egr1 can influence downstream targets reinforcing the pathway's mechanisms in determining cell fate.

Egr1 is linked to cancer and cardiovascular diseases. Abnormal Egr1 expression and activity have been observed in various cancers where it can act as an oncogene or tumor suppressor depending on the context. Abnormal interactions with proteins such as p53 may contribute to tumorigenesis. In cardiovascular diseases Egr1 influences pathological states by regulating genes associated with inflammation and vascular remodeling. The protein’s dysregulation plays a part in atherosclerosis connecting with inflammatory cytokines to promote disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional regulator (PubMed : 20121949). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed : 25258363, PubMed : 25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity). Regulates the amplitude of the expression rhythms of clock genes : BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene BMAL1 in the suprachiasmatic nucleus (SCN) (By similarity).

See full target information EGR1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:347-356 PubMed39866235

2025

EGR1 inhibits clear cell renal cell carcinoma proliferation and metastasis via the MAPK15 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Naixiong Peng,Yuefeng Cai,Dong Chen,Ling Deng,Zejian Zhang,Wei Li

eLife 10: PubMed33780330

2021

The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuko Inoue,Hirofumi Nishizumi,Rumi Ooyama,Kazutaka Mogi,Katsuhiko Nishimori,Takefumi Kikusui,Hitoshi Sakano

Oncology reports 39:1583-1590 PubMed29393474

2018

REC8 inhibits EMT by downregulating EGR1 in gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Junhong Zhao,Lanlan Geng,Gaoyang Duan,Wanfu Xu,Yang Cheng,Zhiliang Huang,Zhenwen Zhou,Sitang Gong

Genes & development 22:1647-61 PubMed18559480

2008

Phosphatidic acid mediates demyelination in Lpin1 mutant mice.

Applications

Species

Mouse

Karim Nadra,Anne-Sophie de Preux Charles,Jean-Jacques Médard,William T Hendriks,Gil-Soo Han,Sandra Grès,George M Carman,Jean-Sébastien Saulnier-Blache,Mark H G Verheijen,Roman Chrast

View all publications

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