AB182624

Anti-Egr1抗体

Name: Egr1抗体 (ab182624)| Abcam中文官网
Brand: Abcam Limited
SKU: AB182624
Price: 4829 CNY
Availability: InStock

Anti-Egr1 antibody

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(4 Publications)

Rabbit Polyclonal EGR1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EGR1 aa 50-350.

查看别名

KROX24, ZNF225, EGR1, Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, NGFI-A

1 Images

Supplier Data

Western blot - Anti-Egr1 antibody (AB182624)

Gel : 8%SDS-PAGE

All lanes:

Western blot - Anti-Egr1 antibody (ab182624) at 1/400 dilution

Lane 1:

RAW264.7 cell line lysate at 50 µg

Lane 2:

NIH 3T3 cell line lysate at 50 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L HRP conjugated antibody at 1/10000 dilution

Predicted band size: 57 kDa

true

Exposure time: 2min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse

应用

applications

免疫原

Recombinant Fragment Protein within Human EGR1 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P18146

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Egr1 also known as early growth response protein 1 is a zinc-finger transcription factor with a molecular weight of approximately 60 kDa. The Egr1 protein plays a significant role in gene expression regulation by binding to specific DNA sequences. This target is highly expressed in a variety of tissues including the brain heart and kidneys. In these tissues Egr1 regulates several cellular processes by activating or repressing target genes. Also Egr1 has monoclonal antibodies available for research purposes providing tools for detailed study in specific cellular contexts.

Biological function summary

Egr1 acts as an immediate-early response gene. It gets rapidly upregulated in response to growth factors stress signals and other extracellular stimuli. As part of the cellular signaling networks Egr1 interacts with other transcription factors to control the expression of genes involved in cell differentiation proliferation and apoptosis. It does not typically form part of a protein complex but may work in concert with other signaling molecules to influence diverse cellular pathways and maintain cellular homeostasis.

Pathways

Egr1 is involved in the MAPK/ERK and PI3K/AKT signaling pathways both key to regulating cellular growth and survival. It functions as a bridging factor that links extracellular signals to transcriptional changes within the nucleus. In the MAPK/ERK pathway Egr1 works alongside proteins like Ras and MEK to promote early gene transcription in response to mitogenic signals. In the PI3K/AKT pathway Egr1 can influence downstream targets reinforcing the pathway's mechanisms in determining cell fate.

Egr1 is linked to cancer and cardiovascular diseases. Abnormal Egr1 expression and activity have been observed in various cancers where it can act as an oncogene or tumor suppressor depending on the context. Abnormal interactions with proteins such as p53 may contribute to tumorigenesis. In cardiovascular diseases Egr1 influences pathological states by regulating genes associated with inflammation and vascular remodeling. The protein’s dysregulation plays a part in atherosclerosis connecting with inflammatory cytokines to promote disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional regulator (PubMed : 20121949). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes (By similarity). Binds double-stranded target DNA, irrespective of the cytosine methylation status (PubMed : 25258363, PubMed : 25999311). Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary (By similarity). Regulates the amplitude of the expression rhythms of clock genes : BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene BMAL1 in the suprachiasmatic nucleus (SCN) (By similarity).

See full target information EGR1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70413 PubMed40384564

2025

Kisspeptin-10 Prevents the Development of Cerebral Aneurysms by Reducing the Expression of Egr-1.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Yu,Minghong Xie,Xuancong Liufu,Yezi Xu,Lei Chen

The Journal of biological chemistry 298:101459 PubMed34864055

2021

Extracellular cathepsin Z signals through the α integrin and augments NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Rhiannon I Campden,Amy L Warren,Catherine J Greene,Jose A Chiriboga,Corey R Arnold,Devin Aggarwal,Neil McKenna,Christina F Sandall,Justin A MacDonald,Robin M Yates

The Journal of biological chemistry 294:6871-6887 PubMed30824540

2019

EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Shi,Yujia Wang,Junzheng Peng,Shijie Qi,Nicolas Vitale,Norio Kaneda,Tomiyasu Murata,Hongyu Luo,Jiangping Wu

Oncology reports 40:1769-1776 PubMed30015900

2018

Suppression of Elk1 inhibits thyroid cancer progression by mediating PTEN expression.

Applications