AB81440

Anti-EGFR (phospho Y992)抗体[EM-12]

Anti-EGFR (phospho Y992) antibody [EM-12]

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(8 Publications)

Mouse Monoclonal EGFR phospho Y992 antibody. Suitable for Flow Cyt (Intra), IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human EGFR phospho Y992.

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

4 Images

Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y992) antibody [EM-12] (AB81440)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y992) antibody [EM-12] (AB81440)

Separation of EGF stimulated A431 cell suspension stained using ab81440 at 1μg/ml (red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).

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Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (AB81440)

Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with ab81440 at 1μg/ml and goat anti-mouse-HRP.

All lanes:

Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)

Predicted band size: 134 kDa

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Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (AB81440)

Immunoprecipitation of EGFR from EGF-treated A431 cells by ab81440. The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.

All lanes:

Immunoprecipitation - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440)

Predicted band size: 134 kDa

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Western blot - Anti-EGFR (phospho Y992) antibody [EM-12] (AB81440)

All lanes:

Western blot - Anti-EGFR (phospho Y992) antibody [EM-12] (ab81440) at 1 µg/mL

Lane 1:

EGF-treated A431 cell line

Lane 2:

EGF-treated CALU-3 cell line

Lane 3:

EGF-treated MCF-7 cell line

Lane 4:

EGF-treated Jurkat cell line

Lane 5:

EGF-treated Ramos cell line

Predicted band size: 134 kDa

Observed band size: 170 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

EM-12

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

WB, IP, Flow Cyt (Intra)

applications

免疫原

Synthetic Peptide within Human EGFR phospho Y992. The exact immunogen used to generate this antibody is proprietary information.

P00533

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-4 µg/mL", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Purified from hybridoma culture supernatant. Purity: > 95% (by SDS-PAGE).

存储溶液

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

The protein expressed by the EGFR gene functions as a receptor tyrosine kinase, binding with ligands from the EGF family to activate several signaling cascades that translate extracellular signals into cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen, BTC/betacellulin, epiregulin, and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation, recruiting adapter proteins like GRB2 and activating complex downstream signaling pathways such as RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC, and STATs, and possibly the NF-kappa-B cascade. EGFR also directly phosphorylates proteins like RGS16 and MUC1, influencing various cellular processes including its coupling with G protein-coupled receptor signaling and cell migration through interaction with CCDC88A/GIV. Isoform 2 of EGFR may act as an antagonist to EGF actions. In microbial infection, EGFR also serves as a receptor facilitating hepatitis C virus (HCV) entry into hepatocytes by promoting CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins. This supplementary information is collated from multiple sources and compiled automatically.

See full target information EGFR phospho Y992

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2130 PubMed38503739

2024

Drug-resistant EGFR mutations promote lung cancer by stabilizing interfaces in ligand-free kinase-active EGFR oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

R Sumanth Iyer,Sarah R Needham,Ioannis Galdadas,Benjamin M Davis,Selene K Roberts,Rico C H Man,Laura C Zanetti-Domingues,David T Clarke,Gilbert O Fruhwirth,Peter J Parker,Daniel J Rolfe,Francesco L Gervasio,Marisa L Martin-Fernandez

Nature cell biology 22:1357-1370 PubMed33139939

2020

Phosphatidylinositol-3-OH kinase signalling is spatially organized at endosomal compartments by microtubule-associated protein 4.

Applications

Unspecified application

Species

Unspecified reactive species

Narendra Thapa,Mo Chen,Hudson T Horn,Suyong Choi,Tianmu Wen,Richard A Anderson

Journal of Cancer 11:3667-3674 PubMed32284763

2020

Cyclooxygenase-2 mediates gefitinib resistance in non-small cell lung cancer through the EGFR/PI3K/AKT axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qin-Fang Deng,Qi-Yu Fang,Xian-Xiu Ji,Song-Wen Zhou

Bioscience reports 40: PubMed32068236

2020

SKA3 promotes lung adenocarcinoma metastasis through the EGFR-PI3K-Akt axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Hu,Hai-Ling Chen,Li-Ming Lou,Hong Zhang,Guo-Liang Yang

The American journal of pathology 189:1637-1653 PubMed31345467

2019

Silencing of LAMC2 Reverses Epithelial-Mesenchymal Transition and Inhibits Angiogenesis in Cholangiocarcinoma via Inactivation of the Epidermal Growth Factor Receptor Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Fei Pei,Jie Liu,Jian Cheng,Wei-Ding Wu,Xi-Qiang Liu

PloS one 12:e0189087 PubMed29253018

2017

New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Klaus Dittmann,Claus Mayer,Stefan Czemmel,Stephan M Huber,H Peter Rodemann

Nature communications 7:13307 PubMed27796308

2016

EGFR oligomerization organizes kinase-active dimers into competent signalling platforms.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah R Needham,Selene K Roberts,Anton Arkhipov,Venkatesh P Mysore,Christopher J Tynan,Laura C Zanetti-Domingues,Eric T Kim,Valeria Losasso,Dimitrios Korovesis,Michael Hirsch,Daniel J Rolfe,David T Clarke,Martyn D Winn,Alireza Lajevardipour,Andrew H A Clayton,Linda J Pike,Michela Perani,Peter J Parker,Yibing Shan,David E Shaw,Marisa L Martin-Fernandez

Breast cancer research : BCR 15:R21 PubMed23497505

2013

Oestrogen increases the activity of oestrogen receptor negative breast cancer stem cells through paracrine EGFR and Notch signalling.

Applications

Species

Human

Hannah Harrison,Bruno M Simões,Lynsey Rogerson,Sacha J Howell,Göran Landberg,Robert B Clarke

View all publications

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