AB5651

Anti-EGFR (phospho Y1148)抗体

Anti-EGFR (phospho Y1148) antibody

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(2 Publications)

Rabbit Polyclonal EGFR phospho Y1148 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EGFR phospho Y1148.

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

2 Images

Supplier Data

Immunocytochemistry - Anti-EGFR (phospho Y1148) antibody (AB5651)

Immunocytochemical analysis of EGFR (pY1148) on A549 cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton™ X-100. The cells were labeled with ab5651 at 1/250 dilution in 1% BSA and incubated for 3 hours at room temperature followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 (Panel a : green). Nuclei (Panel b : blue) were stained DAPI. (Panel c : red) F-actin was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control.

Supplier Data

Western blot - Anti-EGFR (phospho Y1148) antibody (AB5651)

Blocking buffer : 5% BSA-TBST

The phosphopeptide corresponding to EGFR [pY1148] blocks the signal.

All lanes:

Western blot - Anti-EGFR (phospho Y1148) antibody (ab5651) at 1/1000 dilution

Lane 1:

A431 cells unstimulated with no peptide

Lane 2:

A431 cells stimulated with 200 ng/mL EGF for 15 minutes with no peptide

Lane 3:

A431 cells stimulated with 200 ng/mL EGF for 15 minutes with non-phosphopeptide corresponding to the phosphopeptide immunogen

Lane 4:

A431 cells stimulated with 200 ng/mL EGF for 15 minutes with a generic phosphotyrosine-containing peptide

Lane 5:

A431 cells stimulated with 200 ng/mL EGF for 15 minutes with phosphopeptide immunogen

Secondary

All lanes:

goat F(ab’)2 anti-rabbit IgG HRP conjugate

Predicted band size: 134 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC, WB

applications

免疫原

Synthetic Peptide within Human EGFR phospho Y1148. The exact immunogen used to generate this antibody is proprietary information.

P00533

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/250", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

The antibody has been negatively preadsorbed using (i) a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR), and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 1148.

存储溶液

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

See full target information EGFR phospho Y1148

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 125:4529-43 PubMed26571401

2015

PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response.

Applications

Unspecified application

Species

Unspecified reactive species

Hsin-Wei Liao,Jung-Mao Hsu,Weiya Xia,Hung-Ling Wang,Ying-Nai Wang,Wei-Chao Chang,Stefan T Arold,Chao-Kai Chou,Pei-Hsiang Tsou,Hirohito Yamaguchi,Yueh-Fu Fang,Hong-Jen Lee,Heng-Huan Lee,Shyh-Kuan Tai,Mhu-Hwa Yang,Maria P Morelli,Malabika Sen,John E Ladbury,Chung-Hsuan Chen,Jennifer R Grandis,Scott Kopetz,Mien-Chie Hung

Electrophoresis 31:1822-32 PubMed20446292

2010

Indications for cell stress in response to adenoviral and baculoviral gene transfer observed by proteome profiling of human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Gerner,Verena J Haudek-Prinz,Andreas Lackner,Annemarie Losert,Barbara Peter-Vörösmarty,Olga Lorenz,Michael Grusch

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