AB32430

Anti-EGFR (phospho Y1068) 抗体 [Y38]

Anti-EGFR (phospho Y1068) antibody [Y38]

RabMAb
Recombinant
Lab Essentials
20ul selling size
了解详情

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(47 Publications)

Anti-EGFR (phospho Y1068) antibody [Y38] (ab32430) is a rabbit monoclonal antibody detecting EGFR in Western Blot, IP, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
- Trusted since 2006

查看别名

ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [Y38] (AB32430)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [Y38] (AB32430)

Immunocytochemistry/Immunofluorescence analysis of untreated, EGF treated and EGF + LP treated A431 cells labelling EGFR (phospho Y1068) with purified ab32430 (left) and EGFR with ab52894 (right) both at a dilution of 1/1000.

Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

The image shows increased membrane staining after EGF (100ng/ml, 30min) treatment on A431 cells. The LP treatment decreased the increased membrane staining caused by EGF.

ab52894 was used as a Pan control for ab32430. The results showed membrane and cytoplasmic staining on untreated, EGF and EGF + LP treated A431 cells.

Unknown

Immunoprecipitation - Anti-EGFR (phospho Y1068) antibody [Y38] (AB32430)

ab32430 (purified) at 1/30 dilution (2ug) immunoprecipitating EGFR in A431 treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug . A431 (Human epidermoid carcinoma epithelial cell) treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug
Lane 2 (+) : ab32430 & A431 treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32430 in A431 treated with 200 ng/ml EGF for 15 minutes whole cell lysate 10ug
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-EGFR (phospho Y1068) antibody [Y38] (ab32430)

Predicted band size: 134 kDa

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Lab

Western blot - Anti-EGFR (phospho Y1068) antibody [Y38] (AB32430)

All lanes:

Western blot - Anti-EGFR (phospho Y1068) antibody [Y38] (ab32430) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 30 minutes whole cell lysates at 15 µg

Lane 3:

A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 30 minutes. Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

Observed band size: 175 kDa

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不同偶联物与剂型 (3)

Carrier free

Anti-EGFR (phospho Y1068) antibody [Y38] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-EGFR (phospho Y1068) antibody [Y38]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-EGFR (phospho Y1068) antibody [Y38]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

Y38

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody recognises only EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170).

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "For unpurified format use at 1/150 dilution.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "A blocking peptide is available for this antibody as <a href='/products/unavailable/human-egfr-peptide-ab205609'>ab205609</a>.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-EGFR (phospho Y1068) antibody [Y38] (ab32430) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of EGFR?
Anti-EGFR (phospho Y1068) [Y38] (ab32430) specifically detects a band for EGFR (UniProt: P00533) at a molecular weight of 135kDa.

Trusted by the scientific community
Anti-EGFR (phospho Y1068) [Y38] (ab32430) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [Y38] also available for your convenience: ab32430, Carrier free - ab171022, Alexa Fluor® 488 - ab237420, Alexa Fluor® 647 - ab237421

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

EGFR or Epidermal Growth Factor Receptor is a transmembrane glycoprotein that acts as a receptor for members of the epidermal growth factor family. Known alternatively as ErbB1 or HER1 this receptor has an approximate molecular weight of 170 kDa. EGFR is expressed in various cell types notably on epithelial cells and can influence multiple cellular processes through its kinase activity. It participates in the regulation of cell growth multiplication and survival by activating its kinase domain upon ligand binding.

Biological function summary

The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.

Pathways

EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.

EGFR is pertinent to cancer biology including non-small cell lung cancer and glioblastoma where mutations or overexpression of the receptor frequently occur. It connects to proteins such as PTEN and BRAF which influence tumor progression and response to targeted therapies. EGFR's involvement in these disorders highlights its significance as a therapeutic target since it can be manipulated to alter disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins.

See full target information EGFR phospho Y1068

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文献 (47)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:23651 PubMed40603466

2025

TARG1 affects EGFR signaling through the regulation of RNA metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Mihály Mérey,Roberta Fajka-Boja,Gergely Imre,Péter Gudmann,Zsolt Török,Lajos Mátés,Ágnes Czibula,Gyula Timinszky

Oncology research 33:1459-1472 PubMed40486881

2025

Apatinib modulates sorafenib-resistant hepatocellular carcinoma through inhibiting the EGFR/JNK/ERK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dexue Fan,Wei Su,Zhaowen Bi,Xinxing Wang,Xianwen Xu,Mingze Ma,Lichao Zhu,Zhenhai Zhang,Junlin Gao

Life (Basel, Switzerland) 15: PubMed39860044

2025

Two Small Peptides from Hydrolysates Exhibit Antitumor Activity Through Inhibition of TNF-α-Mediated Signal Transduction Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Mengshuang Zhu,Shanshan Zhang,Jiyang Tang,Hairong Hou,Lizhen Wang,Houwen Lin,Xuanming Zhang,Meng Jin

ACS applied materials & interfaces 16:53489-53498 PubMed39348886

2024

Fluidic Interface for Surface-based DNA Origami Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel García-Chamé,Ivy Mayer,Leonie Schneider,Christof M Niemeyer,Carmen M Domínguez

Brain sciences 13: PubMed37759950

2023

The Crosstalk between the EGFR and IFN-γ Pathways and Synergistic Roles in Survival Prediction and Immune Escape in Gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Xingang Zhou,Tingyu Liang,Yulu Ge,Yu Wang,Wenbin Ma

Biology direct 18:33 PubMed37337223

2023

PSMD3-ILF3 signaling cascade drives lung cancer cell proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhang,Qianli Ma,Qiduo Yu,Fei Xiao,Zhenrong Zhang,Hongxiang Feng,Chaoyang Liang

Journal of cell science 136: PubMed36744428

2023

LRRK1 functions as a scaffold for PTP1B-mediated EGFR sorting into ILVs at the ER-endosome contact site.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Hanafusa,Keitaro Fujita,Misa Kamio,Shiori Iida,Yasushi Tamura,Naoki Hisamoto,Kunihiro Matsumoto

Analytical cellular pathology (Amsterdam) 2022:4846336 PubMed36046596

2022

N-Acetyl-L-Cysteine Protects Airway Epithelial Cells during Infection against Mucin Synthesis, Oxidative Stress, and Inflammatory Response and Inhibits HSPA6 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Chi,Yuxia Shan,Zhenze Cui

Frontiers in immunology 13:912095 PubMed35958591

2022

ITGB4 Deficiency in Airway Epithelium Aggravates RSV Infection and Increases HDM Sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Xizi Du,Lin Yuan,Ye Yao,Yu Yang,Kai Zhou,Xinyu Wu,Leyuan Wang,Ling Qin,Wenkai Li,Yang Xiang,Xiangping Qu,Huijun Liu,Xiaoqun Qin,Ming Yang,Chi Liu

International journal of biological sciences 18:349-359 PubMed34975337

2022

ITGB4 deficiency induces mucus hypersecretion by upregulating MUC5AC in RSV-infected airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xizi Du,Yu Yang,Ming Yang,Lin Yuan,Leyuan Wang,Mengping Wu,Kai Zhou,Wenkai Li,Yang Xiang,Xiangping Qu,Huijun Liu,Xiaoqun Qin,Chi Liu

View all publications

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Anti-EGFR (phospho Y1068) 抗体 [Y38]