Anti-EGFR (phospho Y1068) 抗体 [EP774Y]
Anti-EGFR (phospho Y1068) antibody [EP774Y]
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
- 了解详情
4
(14 Reviews)
|
(189 Publications)
Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) is a rabbit monoclonal antibody detecting EGFR in Western Blot, Flow Cytometry (Intra), IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse.
- Biophysical QC for unrivalled batch-batch consistency
- Over 140 publications
- Trusted since 2006
查看别名
ERBB, ERBB1, HER1, EGFR, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
- WB
Lab
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
This antibody detects high background.
All lanes:
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) at 1/1000 dilution
Lane 1:
A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2:
A431 (Human epidermoid carcinoma epithelial cell) treated with 10 ng/ml EGF for 30 minutes whole cell lysates at 15 µg
Lane 3:
A431 (Human epidermoid carcinoma epithelial cell) treated with 10 ng/ml EGF for 30 minutes. Then the membrane was incubated with phosphatase at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 134 kDa
Observed band size: 175 kDa
false
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
Immunofluorescent staining of untreated (C) and Phosphatase- treated (D) A431 cells using ab40815 (unpurified).
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with 200 ng/ml EGF for 15 minutes cells labeling EGFR with purified ab40815 at 1/800 dilution (1 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Untreated A431 cells (Green).
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
WD-PBEC cultures were infected with RSV clinical isolate BT2a and stained for EGFR (red) and RSV F (RSV F, green) expression.
For WD-PBECs, pediatric bronchial epithelial cells (PBEC) were obtained, via written parental consent, from bronchial brushings of children undergoing elective surgery at the Royal Belfast Hospital for Sick Children, and the procedures were approved by the Office of Research Ethics Committees Northern Ireland. PBEC were expanded in collagen-coated flasks using airway epithelial cell media and supplements (Lonza), then seeded onto transwell inserts (Corning), and then air-liquid interface (ALI) cultures were initiated and maintained 21 days in order to establish well-differentiated (WD)-PBECs. Paraformaldehyde-fixed and permeabilized WD-PBEC were stained for RSV F protein expression and were stained with anti-phospho-(p)-EGFR (Abcam, ab40815, unpurified). WD-PBEC cultures were infected with RSV subgroup A clinical isolate BT2a. Fluorescent images were obtained with a SP5 confocal DMI 6000 inverted microscope (Leica).
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) treated with 100 ng/ml EGF for 10 minutes cells labeling EGFR with purified ab40815 at 1 : 500 dilution (1.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling EGFR with purified ab40815 at 1 : 500 dilution (1.75 µg/ml). Heat mediated antigen retrieval was performed using heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- IHC-P
AbReview55755****
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
Formaldehyde-fixed, paraffin-embedded human prostate cancer tissue stained for EGFR (phospho Y1068) using ab40815 (unpurified) at 1/200 dilution in immunohistochemical analysis, followed by Goat anti Rabbit IgG (Biotin).
This image is courtesy of an anonymous Abreview.
- WB
Lab
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
This antibody detects high background.
All lanes:
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) at 1/1000 dilution
Lane 1:
A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2:
A431 (Human epidermoid carcinoma epithelial cell) treated with 10 ng/ml EGF for 30 minutes whole cell lysates at 15 µg
Lane 3:
A431 (Human epidermoid carcinoma epithelial cell) treated with 10 ng/ml EGF for 30 minutes. Then the membrane was incubated with phosphatase at 15 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 134 kDa
Observed band size: 175 kDa
false
- WB
Supplier Data
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
***Blocking and dilution buffer : *** 5% NFDM/TBST.
Lanes 1 - 3:
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) at 1/1000 dilution
Lanes 1 - 3:
Western blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] - BSA and Azide free (<a href='/products/primary-antibodies/egfr-phospho-y1068-antibody-ep774y-bsa-and-azide-free-ab182618'>ab182618</a>) at 1/1000 dilution
Lane 1:
C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg
Lane 2:
C2C12 (Mouse myoblasts myoblast) treated with 100 ng/ml EGF for 24 hours whole cell lysate at 20 µg
Lane 3:
C2C12 (Mouse myoblasts myoblast) treated with 100 ng/ml EGF for 24hours whole cell lysate, then the membrane was incubated with phosphatase at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
false
Exposure time: 30s
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
ab40815 (unpurified) showing positive staining in Glioma tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
ab40815 (unpurified) showing positive staining in Papillary carcinoma of thyroid gland tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
ab40815 (unpurified) showing positive staining in Cervical carcinoma tissue.
- Dot
Unknown
Dot Blot - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
Dot blot analysis of EGFR (pY1068) peptide (Lane 1), SMAD5 (unmodified) peptide labelling EGFR (pY1068) with ab40815 (unpurified) at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
- OI-RD Scanning
Unknown
OI-RD Scanning - Anti-EGFR (phospho Y1068) antibody [EP774Y] (AB40815)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
不同偶联物与剂型 (4)
-
Anti-EGFR (phospho Y1068) antibody [EP774Y] - BSA and Azide free
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-EGFR (phospho Y1068) antibody [EP774Y]
-
665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-EGFR (phospho Y1068) antibody [EP774Y]
-
578 PE
PE Anti-EGFR (phospho Y1068) antibody [EP774Y]
反应性数据
产品详情
Product Specifications
Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Dot Blot, Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse samples.
Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) specifically detects EGFR Phospho-Y1068 (UniProt ID: P00533; Molecular weight: 132kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) has been cited over 140 times in peer reviewed journals and is trusted by the scientific community.
Anti-EGFR (phospho Y1068) antibody [EP774Y] (ab40815) has 12 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone EP774Y - ab182618.
Antibody clone EP774Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, PE (ab205827, ab205828, ab303574).
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The EGFR protein plays an important role in cellular communication and signaling processes. EGFR pairs with other receptor family members to form active dimers or even higher-order complexes which in turn initiate intracellular signaling cascades. Through these complexes EGFR influences many processes including cell differentiation and repair. This function of EGFR makes it an integral part of mammalian biology affecting how cells respond to their environment by mediating changes in gene expression.
Pathways
EGFR is a central player in the MAPK and PI3K/Akt signaling pathways. Alongside other protein partners like KRAS and PI3 kinase it contributes to transmitting signals from the cell surface to the nucleus affecting gene transcription and cell behavior. These pathways are important for normal cell growth and division and aberrations in these pathways can lead to excessive or insufficient cell proliferation.
产品实验方案
- Visit the General protocols
- Visit the Troubleshooting
靶点信息
文献 (189)
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